US 12,306,102 B2
Method and system for quantitative three dimensional measurement of density, anisotropy, and orientation without label
Shalin Mehta, San Francisco, CA (US); Li-Hao Yeh, San Francisco, CA (US); and Ivan Ivanov, San Francisco, CA (US)
Assigned to CZ Biohub SF, LLC, San Francisco, CA (US)
Filed by CZ Biohub SF, LLC, San Francisco, CA (US)
Filed on Nov. 16, 2022, as Appl. No. 18/055,988.
Application 18/055,988 is a continuation of application No. PCT/US2021/034028, filed on May 25, 2021.
Claims priority of provisional application 63/030,841, filed on May 27, 2020.
Prior Publication US 2023/0080237 A1, Mar. 16, 2023
Int. Cl. G01N 21/64 (2006.01); G01N 21/21 (2006.01); G02B 21/16 (2006.01); G02B 21/18 (2006.01); G02B 21/36 (2006.01)
CPC G01N 21/6456 (2013.01) [G01N 21/21 (2013.01); G01N 21/6486 (2013.01); G02B 21/16 (2013.01); G02B 21/18 (2013.01); G02B 21/365 (2013.01); G01N 2201/127 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A system comprising:
an illumination source;
a specimen stage operable to support a specimen;
imaging optics;
a polarization state analyzer optically coupled to the imaging optics; and
one or more processors in communication with the illumination source and the polarization state analyzer, the one or more processors configured to perform operations comprising:
generating a plurality of illumination patterns;
rotating a calibration target at a plurality of orientations for each illumination pattern;
receiving an array of multiple sets of polarization channels from the polarization state analyzer;
converting multiple sets of polarization-resolved images at known orientations of a calibration target into a calibration tensor for each illumination pattern of the plurality of illumination patterns;
converting the array of multiple sets of polarization channels into Stokes parameter maps; and
deconvolving the Stokes parameter maps to provide a volumetric map of the permittivity tensor of the specimen to measure 3D density, 3D anisotropy, and 3D orientation of the specimen.