US 12,306,098 B2
Methods and related aspects for analyzing exosomes
Shaopeng Wang, Chandler, AZ (US); and Pengfei Zhang, Tempe, AZ (US)
Assigned to ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY, Scottsdale, AZ (US)
Filed by ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY, Scottsdale, AZ (US)
Filed on Jun. 1, 2023, as Appl. No. 18/204,735.
Claims priority of provisional application 63/352,929, filed on Jun. 16, 2022.
Prior Publication US 2023/0408409 A1, Dec. 21, 2023
Int. Cl. G01N 21/55 (2014.01); G01N 33/543 (2006.01); G16B 15/00 (2019.01)
CPC G01N 21/55 (2013.01) [G01N 33/54373 (2013.01); G16B 15/00 (2019.02); G01N 2021/556 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method of detecting an exosome, the method comprising:
disposing a fluidic sample that comprises a plurality of exosomes in a chamber that is positioned at least partially within a fluidic device, wherein at least one inner surface of the chamber comprises at least a first set of exosome binding moieties that are capable of binding the exosomes;
binding at least a portion of the plurality of exosomes to at least a portion of the first set of exosome binding moieties to produce one or more surface-bound exosomes;
introducing an incident light toward the inner surface of the chamber prior to, concurrent with, and/or after, producing the surface-bound exosomes; and,
detecting light scattered by the surface-bound exosomes to produce a set of exosome imaging data, thereby detecting the exosome.