	US 12,306,093 B2
	Methods and systems for integrated on-chip single-molecule detection
Pierre Indermuhle, Berkeley, CA (US); Elliott Sorelle, San Carlos, CA (US); David Stern, San Carlos, CA (US); Parag Mallick, San Mateo, CA (US); and Sujal M. Patel, Seattle, WA (US)
Assigned to Nautilus Subsidiary, Inc., Seattle, WA (US)
Filed by Nautilus Subsidiary, Inc., Seattle, WA (US)
Filed on Oct. 28, 2021, as Appl. No. 17/513,877.
Application 17/513,877 is a continuation of application No. PCT/US2020/030501, filed on Apr. 29, 2020.
Claims priority of provisional application 62/840,209, filed on Apr. 29, 2019.
Prior Publication US 2022/0050049 A1, Feb. 17, 2022
Int. Cl. G01N 21/25 (2006.01); C12Q 1/6837 (2018.01); G01N 21/64 (2006.01); G01N 33/543 (2006.01); G01N 21/77 (2006.01)

CPC G01N 21/255 (2013.01) [C12Q 1/6837 (2013.01); G01N 21/6428 (2013.01); G01N 33/54366 (2013.01); G01N 2021/6439 (2013.01); G01N 2021/7793 (2013.01)]

24 Claims

1. A composition, comprising:

a. a substrate;

b. a first structured nucleic acid particle coupled to the substrate, wherein a biological entity is coupled to the first structured nucleic acid particle; and

c. a fluorescent detection agent, wherein the fluorescent detection agent comprises an affinity reagent attached to a second structured nucleic acid particle, wherein the affinity reagent is bound to the biological entity, wherein the first structured nucleic acid particle is coupled to the substrate by binding between a first oligonucleotide and a second oligonucleotide, wherein the first oligonucleotide is attached to the substrate, and wherein the second oligonucleotide comprises a strand of the structured nucleic acid particle.