US 12,305,216 B2
Method for selecting aerobic denitrifying fungus and method for remediating water body with low carbon-to-nitrogen ratio using aerobic denitrifying fungus
Haihan Zhang, Xi'an (CN); Ben Ma, Xi'an (CN); and Limin Niu, Xi'an (CN)
Assigned to XI'AN UNIVERSITY OF ARCHITECTURE AND TECHNOLOGY, Xi'an (CN)
Filed by XI'AN UNIVERSITY OF ARCHITECTURE AND TECHNOLOGY, Xi'an (CN)
Filed on Jun. 6, 2024, as Appl. No. 18/735,209.
Application 18/735,209 is a continuation of application No. PCT/CN2023/132745, filed on Nov. 20, 2023.
Claims priority of application No. 202310160552.8 (CN), filed on Feb. 24, 2023.
Prior Publication US 2024/0309421 A1, Sep. 19, 2024
Int. Cl. C12Q 1/04 (2006.01); C02F 3/34 (2023.01); C02F 101/38 (2006.01); C12N 1/14 (2006.01); C12Q 1/24 (2006.01); C12Q 1/6895 (2018.01); C12R 1/885 (2006.01)
CPC C12Q 1/045 (2013.01) [C02F 3/347 (2013.01); C12N 1/145 (2021.05); C12Q 1/24 (2013.01); C12Q 1/6895 (2013.01); C02F 2101/38 (2013.01); C12R 2001/885 (2021.05)] 4 Claims
 
1. A method for selecting an aerobic denitrifying fungus, comprising:
treating sediments in a reservoir by an ultrasonic shaking technology, comprising subjecting a mud/water mixture collected from a drinking water reservoir to an ultrasonic treatment to obtain a suspension;
isolating and purifying fungal strains and diluting the suspension to obtain a diluted suspension;
coating the diluted suspension on a first fungal solid medium to produce first fungal colonies;
picking fungal colonies with different shapes, colors, and characteristics from the first fungal solid medium; streaking picked fungal colonies on a second fungal solid medium, incubating a streaked fungal solid medium in a biochemical incubator to produce second colonies; and repeating a streaking process a plurality of times until pure colonies are obtained;
selecting a fungus with an aerobic denitrification function using a denitrification medium;
screening the pure colonies obtained after a separation and a purification in the denitrification medium
selecting a fungus with an aerobic denitrification ability; and
identifying a selected fungal strain by a gene sequencing technology, and naming an identified fungal strain as Trichoderma Virens D4.