US 12,305,213 B2
High gravity, fed-batch ionic liquid based process for deconstructing biomass
Feng Xu, Albany, CA (US); Blake Simmons, San Francisco, CA (US); and Seema Singh, Clarksburg, MD (US)
Assigned to National Technology & Engineering Solutions of Sandia, LLC, Albuquerque, NM (US)
Filed by NATIONAL TECHNOLOGY & ENGINEERING SOLUTIONS OF SANDIA, LLC, Albuquerque, NM (US)
Filed on Sep. 13, 2021, as Appl. No. 17/473,906.
Application 17/473,906 is a continuation of application No. 15/777,649, granted, now 11,118,197, issued on Sep. 14, 2021, previously published as PCT/US2016/063195, filed on Nov. 21, 2016.
Claims priority of provisional application 62/257,647, filed on Nov. 19, 2015.
Prior Publication US 2022/0170053 A1, Jun. 2, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 19/02 (2006.01); C12P 7/10 (2006.01); C12P 19/14 (2006.01); C13K 1/02 (2006.01); C13K 13/00 (2006.01)
CPC C12P 7/10 (2013.01) [C12P 19/02 (2013.01); C12P 19/14 (2013.01); C13K 1/02 (2013.01); C13K 13/002 (2013.01); C12P 2201/00 (2013.01); C12P 2203/00 (2013.01); Y02E 50/10 (2013.01)] 7 Claims
 
1. A method of producing a polysaccharide hydrolysate from biomass, the method comprising:
(i) providing a slurry comprising pre-treated biomass at a concentration of at least about 5% w/w and less than about 50% w/w, an ionic liquid or a mixture of ionic liquids at a concentration of between about 5% w/w and about 25% w/w, and water, wherein the ionic liquid or the mixture of ionic liquids and biomass are present in the pre-treated slurry at a mass ratio Rm/i of from about 0.2 to about 5, and wherein the ionic liquid or the mixture of ionic liquids comprises:
a) a choline cation; and
b) an anion selected from the group consisting of a carboxylic acid anion, dicarboxylic acid anion, lysinate, hydroxide anion, bisulfate anion, dihydrogen phosphate anion, phosphate anion, bicarbonate anion, and chloride anion;
(ii) combining a portion of the pre-treated biomass slurry with a glycoside hydrolase and a hydrolysate seed batch comprising a sugar and water, wherein the sugar is at a concentration of at least 70% w/w in the hydrolysate seed batch; and
(iii) maintaining the mixture formed in step (ii) under conditions sufficient to hydrolyze the polysaccharide present in the portion of the pre-treated biomass slurry, thereby forming a mixture comprising a hydrolyzed polysaccharide;
wherein the biomass comprises polysaccharide and lignin, and
wherein at least 70% of glucan and/or xylan present in the biomass is converted into a monosaccharide.