US 12,305,202 B2
Polypeptide composition
Jianwei Zhu, Shanghai (CN); Jing Wang, Shanghai (CN); Lei Han, Shanghai (CN); Junsheng Chen, Shanghai (CN); Yueqing Xie, Shanghai (CN); and Hua Jiang, Shanghai (CN)
Assigned to JECHO LABORATORIES, INC., Frederick, MD (US); and JECHO INSTITUTE, CO., LTD., Shanghai (CN)
Appl. No. 17/280,636
Filed by JECHO INSTITUTE, CO., LTD., Shanghai (CN); and JECHO LABORATORIES, INC., Frederick, MD (US)
PCT Filed Sep. 27, 2019, PCT No. PCT/CN2019/108588
§ 371(c)(1), (2) Date Mar. 26, 2021,
PCT Pub. No. WO2020/063880, PCT Pub. Date Apr. 2, 2020.
Claims priority of application No. 201811162604.0 (CN), filed on Sep. 30, 2018.
Prior Publication US 2022/0282231 A1, Sep. 8, 2022
Int. Cl. C12N 9/14 (2006.01); A61P 35/00 (2006.01); C07K 14/21 (2006.01); C12N 15/11 (2006.01)
CPC C12N 9/14 (2013.01) [A61P 35/00 (2018.01); C07K 14/21 (2013.01); C12N 15/11 (2013.01); C07K 2319/55 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A composition, comprising: a first polypeptide; and a second polypeptide, wherein the first polypeptide comprises a first toxin fragment and a first intein fragment, wherein the second polypeptide comprises a second toxin fragment and a second intein fragment, wherein the first polypeptide is different from the second polypeptide, wherein the first toxin fragment and the second toxin fragment are non-biotoxic, and wherein the first polypeptide and the second polypeptide are capable of making the first toxin fragment and the second toxin fragment to form a toxin with biotoxicity by an interaction between the first intein fragment and the second intein fragment, and wherein the toxin is PE38, which is a truncated form of exotoxin PE from Pseudomonas aeruginosa; wherein the first toxin fragment consists of the amino acid sequence of SEQ ID NO: 4, and the second toxin fragment consists of the amino acid sequence of SEQ ID NO: 14.