US 12,305,194 B2
Cell culture method and cell culture system
Takahiko Totani, Yokohama (JP); Yousuke Matsuoka, Yokohama (JP); and Satoshi Tanaka, Yokohama (JP)
Assigned to TOYO SEIKAN GROUP HOLDINGS, LTD., Tokyo (JP)
Appl. No. 16/486,212
Filed by TOYO SEIKAN GROUP HOLDINGS, LTD., Tokyo (JP)
PCT Filed Jan. 25, 2018, PCT No. PCT/JP2018/002317
§ 371(c)(1), (2) Date Aug. 15, 2019,
PCT Pub. No. WO2018/150841, PCT Pub. Date Aug. 23, 2018.
Claims priority of application No. 2017-029163 (JP), filed on Feb. 20, 2017.
Prior Publication US 2020/0056148 A1, Feb. 20, 2020
Int. Cl. C12N 5/0735 (2010.01); C12N 5/074 (2010.01)
CPC C12N 5/0606 (2013.01) [C12N 5/0696 (2013.01); C12N 2500/30 (2013.01); C12N 2533/52 (2013.01); C12N 2533/90 (2013.01)] 6 Claims
 
1. A cell culture method for adhering cells to a culture container to culture the cells, comprising:
arranging the culture container, wherein at least a part of a culture surface of the culture container is formed of a base material that is any one of polyethylene, cyclic olefin copolymer or polyethylene terephthalate, wherein at least the part of the culture surface is treated using hydrophilization treatment by corona treatment to have a contact angle of not less than 51.0° and not more than 84.0°, and wherein the culture surface is not pre-coated with laminin, and wherein the culture surface is exposed to a medium;
accommodating the medium and the cells in the culture container, wherein the medium comprises laminin or a fragment thereof as a cell adhesion factor; and
culturing the cells so as to absorb the cell adhesion factor on the treated culture surface and adhere the cells to the culture surface which has adsorbed the cell adhesion factor.