	US 12,305,184 B2
	Compositions and methods for conferring resistance to geminivirus
Jose Trinidad Ascencio-Ibanez, Raleigh, NC (US); Linda K. Hanley-Bowdoin, Raleigh, NC (US); and Wei Shen, Morrisville, NC (US)
Assigned to North Carolina State University, Raleigh, NC (US)
Appl. No. 18/688,976
Filed by North Carolina State University, Raleigh, NC (US)
PCT Filed Sep. 6, 2022, PCT No. PCT/US2022/075981 § 371(c)(1), (2) Date Mar. 4, 2024, PCT Pub. No. WO2023/035011, PCT Pub. Date Mar. 9, 2023.
Claims priority of provisional application 63/240,724, filed on Sep. 3, 2021.
Prior Publication US 2024/0271156 A1, Aug. 15, 2024
Int. Cl. C12N 15/82 (2006.01)

CPC C12N 15/8283 (2013.01)

20 Claims

1. A heterologous expression cassette comprising a chimeric nucleotide sequence that is derived from at least two different species, the chimeric nucleotide sequence comprising:

(i) a polynucleotide that encodes a polypeptide that is at least 96% identical to SEQ ID NO: 2;

(ii) operably linked to a promoter that is functional in plants; and

(iii) a transcription terminator,

wherein the promoter, the polynucleotide, and the transcription terminator are not all naturally occurring in a single plant genus, species, accession, or ecotype.