	US 12,305,181 B2
	HPPD variants and methods of use
Fabien Poree, Lyons (FR); Bernd Laber, Idstein (DE); Gudrun Lange, Kelkheim (DE); Manuel Dubald, Raleigh, NC (US); and Roxanne Armstrong, Cary, NC (US)
Assigned to BASF Agricultural Solutions US LLC, Research Triangle Park, NC (US)
Filed by BASF Agricultural Solutions Seed US LLC, Florham Park, NJ (US)
Filed on Aug. 12, 2019, as Appl. No. 16/537,801.
Application 16/537,801 is a division of application No. 15/124,861, granted, now 10,400,249, previously published as PCT/EP2015/054858, filed on Mar. 9, 2015.
Claims priority of provisional application 61/951,039, filed on Mar. 11, 2014.
Claims priority of application No. 14159634 (EP), filed on Mar. 13, 2014.
Prior Publication US 2020/0002715 A1, Jan. 2, 2020
Int. Cl. C12N 15/82 (2006.01); A01N 43/713 (2006.01); C12N 9/02 (2006.01)

CPC C12N 15/8274 (2013.01) [A01N 43/713 (2013.01); C12N 9/0069 (2013.01); C12Y 113/11001 (2013.01)]

13 Claims

1. A recombinant nucleic acid molecule encoding a 4-hydroxyphenylpyruvate dioxygenase (HPPD) protein comprising an amino acid sequence having at least 95% sequence identity to SEQ ID NO: 1, wherein said amino acid sequence comprises a proline at the amino acid position corresponding to amino acid position 335 of SEQ ID NO:1 and a tyrosine at the amino acid position corresponding to amino acid position 336 of SEQ ID NO: 1, and wherein said HPPD protein is tolerant to an HPPD inhibitor herbicide,

wherein the amino acid sequence further comprises

(i) an alanine at the amino acid position corresponding to amino acid position 339 of SEQ ID NO:1; or

(ii) a glutamic acid, or glutamine at the amino acid position corresponding to amino acid position 340 of SEQ ID NO:1.