US 12,305,177 B2
Methods and platforms for sustainable high yield terpenoid production
Natalia Doudareva, West Lafayette, IN (US); Joseph Noel, La Jolla, CA (US); Laura Henry, West Lafayette, IN (US); and Suzanne T. Thomas, La Jolla, CA (US)
Assigned to Purdue Research Foundation, West Lafayette, IN (US); and Salk Institute for Biological Studies, La Jolla, CA (US)
Filed by Purdue Research Foundation, West Lafayette, IN (US); and Salk Institute for Biological Studies, La Jolla, CA (US)
Filed on Dec. 14, 2021, as Appl. No. 17/550,940.
Application 17/550,940 is a continuation of application No. 16/423,017, filed on May 26, 2019, granted, now 11,198,884, issued on Dec. 14, 2021.
Prior Publication US 2022/0098603 A1, Mar. 31, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/82 (2006.01); A01H 6/20 (2018.01)
CPC C12N 15/8216 (2013.01) [A01H 6/20 (2018.05); C12N 15/8243 (2013.01)] 20 Claims
 
1. A plant cell comprising at least a first nucleic acid sequence and a second nucleic acid sequence that are both heterologous to the plant cell, the first nucleic acid sequence encoding 3-hydroxy-3-methylglutaryl CoA reductase (HMGR) and the second nucleic acid sequence encoding either archaeal phosphomevalonate decarboxylase (MPD) or peroxisomal phosphomevalonate kinase (PMK), wherein the transformed plant cell is capable of producing a terpenoid or terpenoid precursor.