	US 12,305,161 B2
	*Method of culturing Haematococcus* species for manufacturing of astaxanthin**
Björn Podola, Cologne (DE); Michael Melkonian, Lohmar (DE); Alice Costa Kiperstok, Cologne (DE); and Petra Sebestyen, Nagyköros (HU)
Assigned to AD ASTRA EHF, Kopavogur (IS)
Filed by AD ASTRA EHF, Kopavogur (IS)
Filed on Jul. 8, 2021, as Appl. No. 17/370,849.
Application 17/370,849 is a continuation of application No. 15/758,143, granted, now 11,085,014, previously published as PCT/EP2016/071270, filed on Sep. 9, 2016.
Claims priority of application No. 15184899 (EP), filed on Sep. 11, 2015.
Prior Publication US 2021/0340489 A1, Nov. 4, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 1/12 (2006.01); C12P 23/00 (2006.01)

CPC C12N 1/12 (2013.01) [C12P 23/00 (2013.01); C12N 2529/10 (2013.01)]

10 Claims

1. A method for an improved astaxanthin production, the method comprising:

providing a substrate;

arranging the Haematococcus species on surface of the substrate to form a biofilm

cultivating the biofilm of Haematococcus species on the substrate at a high light intensity to increase biomass productivity and to induce and to increase astaxanthin production at the same time,

wherein the high light intensity is 250 μmol photons m⁻²s⁻¹or more.