US 12,305,157 B2
System and method for centralized fluid management and culture control
Richard E. Andrews, Manchester, NH (US); Dave D. B. Cannan, Manchester, NH (US); Alekhya Akkapeddi, Manchester, NH (US); Andrew G. Remec, Manchester, NH (US); Bryan A. Finseth, Newbury, NH (US); and Kevin Kim, Hookset, NH (US)
Assigned to DEKA Products Limited Partnership, Manchester, NH (US)
Filed by DEKA Products Limited Partnership, Manchester, NH (US)
Filed on Apr. 28, 2021, as Appl. No. 17/243,324.
Claims priority of provisional application 63/019,260, filed on May 1, 2020.
Prior Publication US 2021/0340486 A1, Nov. 4, 2021
Int. Cl. C12M 1/36 (2006.01); C12M 1/00 (2006.01); C12M 1/34 (2006.01); C12M 3/00 (2006.01)
CPC C12M 41/48 (2013.01) [C12M 23/28 (2013.01); C12M 23/42 (2013.01); C12M 23/44 (2013.01); C12M 27/18 (2013.01); C12M 29/00 (2013.01); C12M 29/20 (2013.01); C12M 41/12 (2013.01); C12M 41/16 (2013.01); C12M 41/32 (2013.01); C12M 41/34 (2013.01)] 10 Claims
OG exemplary drawing
 
1. A method for automatic centralized fluid management and culture control comprising:
(a) automatically, by a system controller, monitoring temperature, nutrient concentration, and dissolved gas concentrations of a cell or tissue culture media;
(b) automatically, by the system controller, maintaining the temperature of the cell or tissue culture media in a media reservoir at a first pre-selected value;
(c) automatically, by the system controller, adjusting the temperature, the nutrient concentration, and the dissolved gas concentrations of the cell or tissue culture media to second pre-selected values prior to delivery to a user-supplied device;
(d) automatically, by the system controller, delivering the temperature, the nutrient concentration, and dissolved gas concentration-adjusted cell or tissue culture media to the user-supplied device;
(e) automatically, by the system controller, recirculating the temperature, the nutrient concentration, and the dissolved gas concentration-adjusted cell or tissue culture media through a flow path including the media reservoir, a reservoir line, a reservoir module cassette, a holding container, a module line, and a bioreactor module cassette within the user-supplied device;
(f) automatically, by the system controller, monitoring the temperature, the nutrient concentration, and the dissolved gas concentrations of the adjusted cell or tissue culture media to verify they remain at the second pre-selected values through the user-supplied device;
(g) if the temperature, or the nutrient concentration, or the dissolved gas concentrations of the adjusted cell or tissue culture media meet the second pre-selected values, automatically, by the system controller, repeating steps (e)-(f); and
(h) if the temperature, or the nutrient concentration, or the dissolved gas concentrations of the adjusted cell or tissue culture media do not meet the second pre-selected values, automatically, by the system controller, delivering the recirculated adjusted cell or tissue culture media to a waste vessel or a sample vessel.