US 12,305,154 B2
Process for microbial synthesis and an apparatus thereof
Prakash Chandra Sahoo, Faridabad (IN); Manoj Kumar, Faridabad (IN); Ravi Prakash Gupta, Faridabad (IN); Suresh Kumar Puri, Faridabad (IN); Deepak Saxena, Faridabad (IN); and Sankara Sri Venkata Ramakumar, Faridabad (IN)
Assigned to Indian Oil Corporation Limited, Mumbai (IN)
Filed by Indian Oil Corporation Limited, Mumbai (IN)
Filed on Sep. 24, 2021, as Appl. No. 17/448,801.
Claims priority of application No. 202021041728 (IN), filed on Sep. 25, 2020.
Prior Publication US 2022/0098537 A1, Mar. 31, 2022
Int. Cl. C12M 1/00 (2006.01); C12M 1/02 (2006.01); C12N 1/20 (2006.01); C12P 7/06 (2006.01); C12P 7/16 (2006.01); C12R 1/425 (2006.01)
CPC C12M 29/06 (2013.01) [C12M 31/00 (2013.01); C12M 41/24 (2013.01); C12N 1/20 (2013.01); C12N 2500/05 (2013.01); C12N 2500/30 (2013.01); C12N 2529/00 (2013.01); C12R 2001/425 (2021.05)] 17 Claims
OG exemplary drawing
 
1. A process for microbial synthesis, the process comprising:
diluting a pre grown inoculum with saline water having ZnSe quantum dots, and a silica gel mixture to prepare an inoculum mixture, wherein the pre grown inoculum comprises microbes, wherein the microbes are selected from the group consisting of Enterobacter aerogenes MTCC 25016, Serratia sp. MTCC 25017, Shewanella sp. MTCC 25020 and Alcaligenes sp. MTCC 25022;
spraying the inoculum mixture on a biofilm support placed in a mist bioreactor to form a biofilm;
adding a nutrient mist medium, a homoserine lactone, and dioleoylphosphatydic acid and mechanically stirring for about 30 minutes to form a homogeneous mixture;
sending the homogeneous mixture to a mist generation chamber to form an ultra-fine mist, wherein the ultra-fine mist is formed by ultra sonication;
passing the ultra-fine mist through a UV chamber for a duration of 2-5 seconds;
sparging nitrogen gas along with the ultra-fine mist into the mist bioreactor; wherein the ratio of the ultra-fine mist and the nitrogen gas is 1:2;
passing a feed through the mist bioreactor having the biofilm and collecting a product in a product collection and separation chamber.