US 12,304,974 B2
Hyaluronic acid-collagen copolymer compositions and medical applications thereof
Dale P. DeVore, Chelmsford, MA (US); and Jiaxun Zhu, Shanghai (CN)
Assigned to Shanghai Qisheng Biological Preparation Co., Ltd., Shanghai (CN)
Filed by Shanghai Qisheng Biological Preparation Co., Ltd., Shanghai (CN)
Filed on Dec. 28, 2022, as Appl. No. 18/089,788.
Claims priority of provisional application 63/294,164, filed on Dec. 28, 2021.
Prior Publication US 2023/0203207 A1, Jun. 29, 2023
Int. Cl. A61K 31/728 (2006.01); A61K 31/726 (2006.01); A61K 31/727 (2006.01); C08B 37/00 (2006.01); C08B 37/08 (2006.01); C08B 37/10 (2006.01); C08G 81/00 (2006.01); C12P 13/08 (2006.01)
CPC C08B 37/0063 (2013.01) [C12P 13/08 (2013.01)] 21 Claims
 
1. A method for preparing a glycosaminoglycan-soluble collagen copolymer comprising the steps of:
a) chemically grafting poly-L-lysine (PLL) to the glycosaminoglycan by reacting the glycosaminoglycan with PLL to produce a glycosaminoglycan-PLL gel;
b) chemically derivatizing soluble collagen by reacting a collagen solution at an alkaline pH with an acetylation agent to produce a collagen gel derivative;
c) mixing the glycosaminoglycan-PLL gel with the collagen gel derivative; and
d) adding a bifunctional acetylation agent to react with free amine groups on glycosaminoglycan-PLL and collagen gel derivative to produce a mixture of glycosaminoglycan-PLL-collagen gel, glycosaminoglycan-PLL polymer, and collagen gel derivative polymer,
wherein the glycosaminoglycan is selected from hyaluronic acid, heparosan, a salt of hyaluronic acid, a salt of heparosan, and combinations thereof.