	US 12,304,927 B2
	De novo design of protein switches
Robert A. Langan, Seattle, WA (US); Scott Boyken, Seattle, WA (US); David Baker, Seattle, WA (US); Walter Novak, Seattle, WA (US); Marc Joseph Lajoie, Seattle, WA (US); and Alfredo Quijano Rubio, Seattle, WA (US)
Assigned to UNIVERSITY OF WASHINGTON, Seattle, WA (US)
Filed by UNIVERSITY OF WASHINGTON, Seattle, WA (US)
Filed on Oct. 22, 2020, as Appl. No. 17/077,692.
Application 17/077,692 is a continuation of application No. 16/796,009, filed on Feb. 20, 2020, granted, now 10,851,135.
Application 16/796,009 is a continuation of application No. PCT/US2019/042651, filed on Jul. 19, 2019.
Claims priority of provisional application 62/788,398, filed on Jan. 4, 2019.
Claims priority of provisional application 62/785,537, filed on Dec. 27, 2018.
Claims priority of provisional application 62/700,681, filed on Jul. 19, 2018.
Prior Publication US 2021/0115090 A1, Apr. 22, 2021
Int. Cl. C07K 14/00 (2006.01); A61K 47/69 (2017.01)

CPC C07K 14/001 (2013.01) [A61K 47/6949 (2017.08); C07K 2319/40 (2013.01); C07K 2319/60 (2013.01); C07K 2319/70 (2013.01)]

10 Claims

1. A LOCKR switch comprising

(a) a cage polypeptide comprising

the amino acid sequence of SEQ ID NO: 27194, wherein the C-terminal 60 amino acids are optional and may be present or may be deleted;

and

(b) a key polypeptide comprising the amino acid sequence of SEQ ID NO: 27195.