	US 12,304,848 B2
	Pseudomonas stutzeri strain, composite microbial inoculum prepared from Pseudomonas stutzeri strain and use of composite microbial inoculum
Xuxiang Zhang, Jiangsu (CN); Kailong Huang, Jiangsu (CN); Lin Ye, Jiangsu (CN); and Hongqiang Ren, Jiangsu (CN)
Assigned to NANJING UNIVERSITY, Jiangsu (CN)
Appl. No. 17/623,257
Filed by NANJING UNIVERSITY, Jiangsu (CN)
PCT Filed Oct. 30, 2019, PCT No. PCT/CN2019/114172 § 371(c)(1), (2) Date Dec. 28, 2021, PCT Pub. No. WO2021/077453, PCT Pub. Date Apr. 29, 2021.
Claims priority of application No. 201911015530.2 (CN), filed on Oct. 24, 2019.
Prior Publication US 2022/0356098 A1, Nov. 10, 2022
Int. Cl. C02F 3/02 (2023.01); C02F 3/34 (2023.01); C12N 1/20 (2006.01); C02F 103/06 (2006.01); C12R 1/38 (2006.01)

CPC C02F 3/341 (2013.01) [C02F 3/02 (2013.01); C12N 1/205 (2021.05); C02F 2103/06 (2013.01); C12R 2001/38 (2021.05)]

5 Claims

1. A preparation method for a composite microbial inoculum, comprising:

(1) inoculating Pseudomonas balearica EBT-1 with Deposit No. CCTCC M 2019730 and Pseudomonas stutzeri EBT-2 with Deposit No. CCTCC M 2019731 separately into a culture medium, and incubating in an aerobic condition at 30-35° C. for 24-32 h to give an EBT-1 expansion product and an EBT-2 expansion product, respectively; and

(2) mixing the EBT-1 expansion product and the EBT-2 expansion product in a volume ratio of 1:1 to give the composite microbial inoculum.