US 12,303,899 B2
Systems and methods for on-chip analysis of nucleic acids and for multiplexed analysis of cells
Harold G. Craighead, Ithaca, NY (US); Harvey C. Tian, Ithaca, NY (US); David M. Lin, Ithaca, NY (US); and Adam J. Bisogni, Ithaca, NY (US)
Assigned to CORNELL UNIVERSITY, Ithaca, NY (US)
Appl. No. 17/298,507
Filed by CORNELL UNIVERSITY, Ithaca, NY (US)
PCT Filed Nov. 29, 2019, PCT No. PCT/US2019/063887
§ 371(c)(1), (2) Date May 28, 2021,
PCT Pub. No. WO2020/113192, PCT Pub. Date Jun. 4, 2020.
Claims priority of provisional application 62/772,618, filed on Nov. 28, 2018.
Claims priority of provisional application 62/772,620, filed on Nov. 28, 2018.
Prior Publication US 2022/0016629 A1, Jan. 20, 2022
Int. Cl. B01L 3/00 (2006.01); C12Q 1/6837 (2018.01)
CPC B01L 3/502761 (2013.01) [C12Q 1/6837 (2013.01); B01L 2200/0668 (2013.01); B01L 2300/0819 (2013.01); B01L 2300/0887 (2013.01); B01L 2300/123 (2013.01); B01L 2400/086 (2013.01)] 20 Claims
OG exemplary drawing
 
1. An integrated microfluidic cell processing system comprising:
a flow directing system comprising a plurality of reconfigurable microfluidic layers that include:
an affinity microcolumn layer comprising a plurality of substantially vertical microcolumns passing therethrough and containing an affinity chromatography agent; and
at least one microfluidic chip functionally integrated into a layer below the affinity microcolumn layer, wherein said at least one microfluidic chip comprises a cell capture component and a nucleic acid entanglement component,
wherein the system operates under continuous flow conditions to process one or more cell.