US 12,303,892 B2
Devices and methods for analyzing biological samples
Tarun Kumar Khurana, Palo Alto, CA (US); Ali Agah, Palo Alto, CA (US); Yir-Shyuan Wu, Palo Alto, CA (US); and Filiz Gorpe Yasar, Palo Alto, CA (US)
Assigned to Cellanome, Inc., Foster City, CA (US)
Filed by Cellanome, Inc., Foster City, CA (US)
Filed on Jul. 17, 2024, as Appl. No. 18/776,193.
Application 18/776,193 is a continuation of application No. 18/593,787, filed on Mar. 1, 2024, granted, now 12,151,242.
Application 18/593,787 is a continuation of application No. 18/219,545, filed on Jul. 7, 2023, granted, now 12,030,047, issued on Jul. 9, 2024.
Application 18/219,545 is a continuation of application No. PCT/US2022/011720, filed on Jan. 7, 2022.
Claims priority of provisional application 63/253,500, filed on Oct. 7, 2021.
Claims priority of provisional application 63/135,463, filed on Jan. 8, 2021.
Prior Publication US 2024/0367167 A1, Nov. 7, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/68 (2018.01); B01L 3/00 (2006.01); C12Q 1/6869 (2018.01); C12Q 1/6874 (2018.01); G01N 33/543 (2006.01); G01N 33/68 (2006.01)
CPC B01L 3/502715 (2013.01) [B01L 3/502707 (2013.01); B01L 3/502761 (2013.01); C12Q 1/6869 (2013.01); C12Q 1/6874 (2013.01); G01N 33/54386 (2013.01); G01N 33/6842 (2013.01); B01L 2200/0647 (2013.01); B01L 2200/12 (2013.01); B01L 2300/0663 (2013.01); B01L 2300/0883 (2013.01); B01L 2300/12 (2013.01); B01L 2300/163 (2013.01); B01L 2300/18 (2013.01); C12Q 2600/158 (2013.01); G01N 2570/00 (2013.01)] 28 Claims
 
1. A method, comprising:
(a) introducing a plurality of cells into a fluidic device, wherein the fluidic device comprises a surface comprising one or more capture elements, and wherein a cell of the plurality of cells couples to a capture element of the one or more capture elements;
(b) introducing one or more polymer precursors into the fluidic device; and
(c) selectively applying light to the fluidic device to polymerize the one or more polymer precursors, thereby selectively encapsulating the cell of the plurality of cells in the fluidic device.