US 12,303,616 B2
Anti-thrombotic and endothelialization-enhanced bioprosthetic valve material and preparation method thereof
Li Yang, Chengdu (CN); Yunbing Wang, Chengdu (CN); Rifang Luo, Chengdu (CN); and Xingdong Zhang, Chengdu (CN)
Assigned to Sichuan University, Chengdu (CN)
Filed by Sichuan University, Chengdu (CN)
Filed on Apr. 21, 2021, as Appl. No. 17/235,952.
Claims priority of application No. 202010317451.3 (CN), filed on Apr. 21, 2020.
Prior Publication US 2021/0322641 A1, Oct. 21, 2021
Int. Cl. A61L 27/36 (2006.01); A61L 27/24 (2006.01)
CPC A61L 27/3687 (2013.01) [A61L 27/24 (2013.01); A61L 27/3625 (2013.01); A61L 2300/802 (2013.01); A61L 2430/20 (2013.01); A61L 2430/40 (2013.01)] 6 Claims
OG exemplary drawing
 
1. A preparation method of an anti-thrombotic and surface endothelialization bioprosthetic valve material, comprising the following steps:
immersing a glutaraldehyde cross-linked bioprosthetic valve material in a 30 mg/mL recombinant human type III collagen solution for 1-24 hours to obtain a first soaked material, taking the first soaked material out of the solution and cleaning the first soaked material with deionized water to obtain a deionized water-cleaned material, wherein the recombinant human type III collagen in the recombinant human type III collagen solution is hydroxyproline-free and does not comprise collagen-mimetic peptides as shown in SEQ ID NO: 2,
wherein the glutaraldehyde cross-linked bioprosthetic valve material is prepared by immersing an animal-derived pericardial biomaterial in an aqueous solution or phosphate buffer saline (PBS) with a volume concentration of 0.1-10% glutaraldehyde for 4-38 hours, and
wherein the animal-derived pericardial biomaterial consists of sheep pericardium.