US 12,303,615 B2
Demineralized bone paper
Jungwoo Lee, Boston, MA (US); Jaehyuck Shim, Boston, MA (US); Ryan Carpenter, Boston, MA (US); Yongkuk Park, Boston, MA (US); and Jun-Goo Kwak, Boston, MA (US)
Assigned to University of Massachusetts, Boston, MA (US)
Filed by UNIVERSITY OF MASSACHUSETTS, Boston, MA (US)
Filed on Nov. 19, 2021, as Appl. No. 17/531,328.
Claims priority of provisional application 63/115,735, filed on Nov. 19, 2020.
Prior Publication US 2022/0152274 A1, May 19, 2022
Int. Cl. A61L 27/00 (2006.01); A61L 27/36 (2006.01); C12M 1/00 (2006.01); C12M 1/32 (2006.01); C12N 5/077 (2010.01); G01N 1/28 (2006.01); G01N 33/483 (2006.01)
CPC A61L 27/3608 (2013.01) [A61L 27/3683 (2013.01); C12M 23/12 (2013.01); C12M 23/22 (2013.01); C12N 5/0669 (2013.01); G01N 1/2813 (2013.01); G01N 33/4833 (2013.01); A61L 2300/414 (2013.01); A61L 2430/02 (2013.01); C12N 2527/00 (2013.01)] 6 Claims
OG exemplary drawing
 
1. A method for assaying the effect of an agent on bone development or health, comprising
(a) providing an assay system comprising
(i) a multi-well culture plate,
(ii) one or more layers of semi-transparent, demineralized, compact bone slices 10-1,000 μm thick in one or more wells of the multi-well culture plate, further comprising bone-promoting cells on the one or more layers, wherein the compact bone slices have been remineralized by the bone-promoting cells, and
(iii) a culture medium configured to promote or maintain bone development;
(b) adding a candidate agent to the culture medium of the assay system; and
(c) measuring at least one parameter of bone development in the assay system to determine if the candidate agent affected bone development, bone resorption, or health.