US 12,303,596 B2
Thermo-responsive hydrogel for intratumoral administration as a treatment in solid tumor cancers
Helena Kelly, Dublin (IE); Garry Duffy, Dublin (IE); Seona Rossi, Dublin (IE); and Conn Hastings, Dublin (IE)
Assigned to THE ROYAL COLLEGE OF SURGEONS IN IRELAND, Dublin (IE)
Filed by Royal College of Surgeons in Ireland, Dublin (IE)
Filed on Nov. 2, 2022, as Appl. No. 17/979,506.
Application 17/979,506 is a continuation of application No. 16/762,138, granted, now 11,534,401, previously published as PCT/EP2018/080518, filed on Nov. 7, 2018.
Claims priority of application No. 17200449 (EP), filed on Nov. 7, 2017.
Prior Publication US 2023/0126053 A1, Apr. 27, 2023
Int. Cl. A61K 9/06 (2006.01); A61K 9/00 (2006.01); A61K 9/19 (2006.01); A61K 31/337 (2006.01); A61K 31/352 (2006.01); A61K 31/7068 (2006.01); A61K 33/243 (2019.01); A61K 47/00 (2006.01); A61K 47/10 (2017.01); A61K 47/22 (2006.01); A61K 47/36 (2006.01); A61K 47/38 (2006.01); A61K 47/40 (2006.01)
CPC A61K 9/06 (2013.01) [A61K 9/0024 (2013.01); A61K 9/19 (2013.01); A61K 31/337 (2013.01); A61K 31/352 (2013.01); A61K 31/7068 (2013.01); A61K 33/243 (2019.01); A61K 47/00 (2013.01); A61K 47/10 (2013.01); A61K 47/22 (2013.01); A61K 47/36 (2013.01); A61K 47/38 (2013.01); A61K 47/40 (2013.01)] 14 Claims
 
1. A method of making a thermo-responsive hydrogel comprising the steps of:
providing a first solution of a cyclodextrin and chitosan in an aqueous base;
cooling the first solution to less than 10° C.;
adding a poloxamer to the first solution and mixing at a temperature of less than 10° C.;
storing the solution at a temperature of less than 10° C. for at least four hours to allow the solution to hydrate;
adding genipin to the hydrated solution with stirring for a period of at least four hours to form the thermo-responsive hydrogel; and
optionally, lyophilising the thermo-responsive hydrogel,
in which the method does not include a step of curing the hydrogel.