US 12,303,233 B2
Devices, systems, and methods for virtual staining
Peter Bryant-Greenwood, Honolulu, HI (US); Kevin P. Rosenblatt, Houston, TX (US); and Jeffrey N. Yu, Honolulu, HI (US)
Assigned to Genetic Innovations, Inc., Honolulu, HI (US)
Filed by Genetic Innovations, Inc., Honolulu, HI (US)
Filed on Jun. 7, 2023, as Appl. No. 18/331,108.
Application 18/331,108 is a continuation of application No. 16/911,098, filed on Jun. 24, 2020, granted, now 11,684,264.
Application 16/911,098 is a continuation of application No. 14/224,903, filed on Mar. 25, 2014, granted, now 10,729,325, issued on Aug. 4, 2020.
Application 14/224,903 is a continuation of application No. 13/843,588, filed on Mar. 15, 2013, granted, now 8,725,237, issued on May 13, 2014.
Claims priority of provisional application 61/656,930, filed on Jun. 7, 2012.
Claims priority of provisional application 61/612,925, filed on Mar. 19, 2012.
Prior Publication US 2023/0404406 A1, Dec. 21, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. A61B 5/00 (2006.01); G06T 5/00 (2024.01); G06T 5/90 (2024.01); G06T 7/00 (2017.01); G06T 7/90 (2017.01); A61B 5/055 (2006.01); G01R 33/56 (2006.01)
CPC A61B 5/0059 (2013.01) [G06T 5/90 (2024.01); G06T 7/0012 (2013.01); G06T 7/90 (2017.01); A61B 5/055 (2013.01); A61B 2576/00 (2013.01); G01R 33/5608 (2013.01); G06T 2207/10024 (2013.01); G06T 2207/10056 (2013.01); G06T 2207/30024 (2013.01)] 20 Claims
OG exemplary drawing
 
11. A method for virtually staining a tissue sample, the method comprising:
accessing an electronic medical image of the tissue sample, the electronic medical image of the tissue sample comprising a plurality of pixels generated based on electromagnetic radiation detected by a detector, wherein the plurality of pixels comprises a first pixel and a second pixel;
determining that a first waveform signature associated with the first pixel of the plurality of pixels is based on a combination of a first plurality of waveform signatures corresponding to multiple substances in the tissue sample at a location associated with the first pixel;
determining the first plurality of waveform signatures;
applying transformation data to the first plurality of waveform signatures in order to obtain a first plurality of outputs;
determining a first single output pixel by volume averaging the first plurality of outputs;
generating a virtually stained first input image of the tissue sample, wherein the virtually stained first input image comprises at least the first single output pixel;
determining that a second waveform signature associated with the second pixel of the plurality of pixels is based on a combination of a second plurality of waveform signatures corresponding to multiple substances in the tissue sample at a location associated with the second pixel;
determining the second plurality of waveform signatures;
applying transformation data to the second plurality of waveform signatures in order to obtain a second plurality of outputs;
determining a second single output pixel by volume averaging the second plurality of outputs;
generating a virtually stained second input image of the tissue sample, wherein the virtually stained second input image comprises at least the second single output pixel;
generating a combined virtually stained image of the tissue sample comprising the virtually stained first input image and the virtually stained second input image; and
displaying the combined virtually stained image of the tissue sample, wherein the combined virtually stained image is substantially identical to an image of the tissue sample when the tissue sample is treated with an actual stain that corresponds to a virtual stain defined by a virtual staining transform.