| CPC A61B 34/20 (2016.02) [A61B 17/3403 (2013.01); A61M 5/427 (2013.01); A61B 2034/2055 (2016.02)] | 17 Claims |

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1. A trans-illuminating needle guide device for guiding a needle into and illuminating an arteriovenous (AV) fistula or AV graft in a subject, comprising:
a housing comprising a base;
a needle guide insert having a pair of opposing side walls defining a first angled slot, a second angled slot, and a plurality of grooves, the needle guide insert configured to be removably coupled to the base;
a needle guide having a guide channel configured to receive the needle, a first deflectable plate configured to be received by the first angled slot and the plurality of grooves, and a second deflectable plate configured to be received by the second angled slot;
a first plurality of light emitting diodes (LEDs) disposed within the housing and configured to emit light of a first wavelength; and
a second plurality of LEDs disposed within the housing and configured to emit light of a second wavelength, the second wavelength differing from the first wavelength,
wherein each groove of the plurality of grooves corresponds to a different needle insertion depth, and
wherein the first and second pluralities of LEDs are configured to illuminate the AV fistula or AV graft or the AV graft of the subject.
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