US 11,984,197 B2
Microbial quantitation
Ariel David Zajdband, Berkeley, CA (US); Wyatt Hugh Hartman, San Francisco, CA (US); David Curtis Stone, San Francisco, CA (US); and Rosaria Chiang, Redwood City, CA (US)
Assigned to TRACE GENOMICS, INC., Ames, IA (US)
Filed by Trace Genomics, Inc., Burlingame, CA (US)
Filed on Jun. 3, 2020, as Appl. No. 16/892,093.
Claims priority of provisional application 62/856,429, filed on Jun. 3, 2019.
Prior Publication US 2020/0381081 A1, Dec. 3, 2020
Int. Cl. C12Q 1/6869 (2018.01); C12N 1/06 (2006.01); C12Q 1/06 (2006.01); G01N 33/24 (2006.01); G16B 30/00 (2019.01); G16B 35/20 (2019.01)
CPC G16B 30/00 (2019.02) [C12N 1/06 (2013.01); C12Q 1/06 (2013.01); C12Q 1/6869 (2013.01); G01N 33/24 (2013.01); G16B 35/20 (2019.02); G01N 2033/243 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A system comprising:
a sampling tube for obtaining a soil sample to which is added one or more synthetic nucleic acids; and
a non-transitory computer readable storage medium having instructions that when executed by one or more processors cause the one or more processors to:
determine a plurality of sequence reads of the soil sample;
classify the plurality of sequence reads into one or more subsets, each subset matched to a particular microbe present in the soil sample;
assign to the soil sample a soil texture label according to one or more measures of texture of soil in the soil sample;
determine a measure of an amount of a microbe within the soil sample as a function of at least a subset of the plurality of sequence reads matched to the microbe, the soil texture label assigned, and a mass of the one or more synthetic nucleic acids present in the soil sample; and
transmit the measure of the microbe within the soil sample to a client device for display on a user interface.