US 11,982,680 B2
Devices, systems, and methods for measuring biomarkers in biological fluids
Daniel B. Lookadoo, Boston, MA (US); Ashok A. Kumar, Malden, MA (US); Jeremy E. Schonhorn, Hopkinton, MA (US); Christopher M. Uherek, Boston, MA (US); and Michal Depa, Beaconsfield (CA)
Assigned to Jana Care, Inc., Watertown, MA (US)
Filed by Jana Care, Inc., Boston, MA (US)
Filed on Dec. 9, 2022, as Appl. No. 18/078,688.
Application 18/078,688 is a continuation of application No. 16/818,976, filed on Mar. 13, 2020, granted, now 11,536,732.
Prior Publication US 2023/0176079 A1, Jun. 8, 2023
Int. Cl. G01N 33/70 (2006.01); G01N 33/535 (2006.01); G01N 33/68 (2006.01); G01N 35/00 (2006.01); G16C 20/10 (2019.01); H04W 84/18 (2009.01)
CPC G01N 33/70 (2013.01) [G01N 33/535 (2013.01); G01N 33/6893 (2013.01); G01N 35/00069 (2013.01); G16C 20/10 (2019.02); H04W 84/18 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A system for measuring a concentration of a biomarker in a sample of biological fluid using an optoelectronic reader coupled to a mobile device having a central processing unit, the system comprising:
a biosensor including—
a top layer;
a bottom layer; and
an active enzymatic component assembly positioned between the top and bottom layers, the assembly including—
an interference mitigation layer positioned below the top layer, the interference mitigation layer being configured to receive the sample, and to enzymatically deplete or remove endogenous substance(s) therefrom, and
a detection layer fluidly coupled to the interference mitigation layer to receive at least a portion of the sample therefrom, the detection layer being configured to enzymatically produce an optical response proportional to the concentration of the biomarker in the portion of the sample of the biological fluid, wherein the biosensor is configured to be positioned within an optoelectronic reader for detection of the optical response; and
an optoelectronic reader configured to be coupled to the mobile device, the optoelectronic reader including—
structure defining an aperture configured to permit light to pass into and out of the optoelectronic reader at a defined viewing axis,
structure defining a slot for operable insertion of the biosensor, the slot being configured to orient the biosensor to intersect the defined viewing axis,
a microcontroller configured to control electronic components on a circuit within the optoelectronic reader and to operably communicate with the central processing unit of the mobile device,
at least one light source configured to emit light at a wavelength in a range from about 400 nm to about 700 nm,
an optical system configured to detect optical signals from the biosensor, and
a power source disposed within the optoelectronic reader and operatively coupled with the microcontroller, the at least one light source, and the optical system,
wherein the optoelectronic reader is configured to communicate signals representative of optical signals from the optical system to the central processing unit of the mobile device to be analyzed to provide an estimate of a concentration of the biomarker in the portion of the sample of the biological fluid in which endogenous substance(s) have been removed.