US 11,982,671 B2
Identification of a T cell epitope of Prevotella copri that induces T cell responses in patients with rheumatoid arthritis
Allen C. Steere, Wellesley, MA (US); and Elise E. Drouin, Boston, MA (US)
Assigned to THE GENERAL HOSPITAL CORPORATION, Boston, MA (US)
Filed by THE GENERAL HOSPITAL CORPORATION, Boston, MA (US)
Filed on Dec. 9, 2020, as Appl. No. 17/116,260.
Application 17/116,260 is a continuation of application No. 16/307,055, granted, now 10,908,159, previously published as PCT/US2017/036198, filed on Jun. 6, 2017.
Claims priority of provisional application 62/346,666, filed on Jun. 7, 2016.
Prior Publication US 2021/0247391 A1, Aug. 12, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/569 (2006.01); C07K 16/12 (2006.01); G01N 33/533 (2006.01); G01N 33/564 (2006.01); G01N 33/68 (2006.01)
CPC G01N 33/564 (2013.01) [C07K 16/1257 (2013.01); G01N 33/533 (2013.01); G01N 33/56916 (2013.01); G01N 33/6878 (2013.01); C07K 2317/34 (2013.01); G01N 2469/20 (2013.01); G01N 2800/102 (2013.01)] 14 Claims
 
1. A method for determining whether a biological sample obtained from a subject is immunologically reactive with Prevotella copri (P. copri) Pc-p27 protein or antigen thereof, the method comprising:
(a) performing an assay for identifying the presence of antibodies that specifically bind the P. copri Pc-p27 protein or antigen thereof using a kit comprising one or more polypeptides comprising an amino acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, and combinations thereof, and reagents for conducting an assay for detecting the presence of an antibody in a sample that binds to the one or more polypeptides, wherein the one or more polypeptides are attached to a support matrix or further comprise a detectable marker; or
(b) performing an assay for identifying T cells specifically reactive to the P. copri Pc-p27 protein or antigen thereof using a composition comprising one or more polypeptides comprising an amino acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, and combinations thereof; and
(c) determining the biological sample is reactive with the P. copri Pc-p27 protein or antigen thereof when either assay produces positive results compared to an appropriate control sample.