US 11,982,662 B2
Marker for screening and identifying not-from-concentrate apple juice based on non-targeted metabolomics and use thereof
Zhenzhen Xu, Beijing (CN); Lei Xu, Beijing (CN); Jiming Zhang, Beijing (CN); Chuanzhu Leng, Beijing (CN); Xue Wang, Beijing (CN); Kewen Wang, Beijing (CN); Gang Xin, Beijing (CN); and Xinfei Song, Beijing (CN)
Filed by INSTITUTE OF QUALITY STANDARD AND TESTING TECHNOLOGY FOR AGRO PRODUCTS OF CAAS, Beijing (CN); and SDIC ZHONGLU FRUIT JUICE CO., LTD., Beijing (CN)
Filed on Mar. 25, 2021, as Appl. No. 17/212,204.
Claims priority of application No. 202011490243.X (CN), filed on Dec. 16, 2020.
Prior Publication US 2022/0187267 A1, Jun. 16, 2022
Int. Cl. G01N 33/02 (2006.01)
CPC G01N 33/025 (2013.01) 6 Claims
OG exemplary drawing
 
1. A method for distinguishing NFC apple juice and FC apple juice, wherein the method comprises:
detecting a content of at least one marker in an apple juice sample to be distinguished; and judging whether the apple juice sample to be distinguished is NFC apple juice or FC apple juice according to the content of the marker,
wherein the judging comprises: substituting the content of at least one marker of the apple juice sample to be distinguished into a distinguishing model for distinguishing, wherein the distinguishing model is a PLS-DA model constructed according to contents of the at least one marker in an NFC apple juice sample and a FC apple juice sample,
wherein contents of the at least one marker in the sample to be distinguished, the NFC apple juice sample and the FC apple juice sample are detected by a chromatographic technique, a mass-spectrometric technique or a combination thereof,
wherein the PLS-DA model is established according to content data of the at least one marker accumulated in a positive ion mode, and
wherein the at least one marker is at least one selected from following markers: gallocatechin, catechin, p-hydroxybenzaldehyde, caffeic acid, chlorogenic acid, epicatechin, isoquercitrin, n-fructosyl isoleucine, p-coumaric acid, phloretin, phlorizin, procyanidin B1, quercetin-3-O-galactoside and rutin.