US 11,982,619 B2
Method for detecting biomaterial using linear upconversion fluorescent property
Sehoon Kim, Seoul (KR); Bong Hyun Chung, Seoul (KR); Youngsun Kim, Seoul (KR); Kyung Mi Park, Daejeon (KR); and Young Hun Seo, Seoul (KR)
Assigned to BIONANO HEALTH GUARD RESEARCH CENTER, Daejeon (KR); and KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY, Seoul (KR)
Filed by Bionano Health Guard Research Center, Daejeon (KR); and Korea Institute of Science and Technology, Seoul (KR)
Filed on Jul. 22, 2020, as Appl. No. 16/935,687.
Application 16/935,687 is a division of application No. 15/574,392, granted, now 10,761,025, previously published as PCT/KR2016/005166, filed on May 16, 2016.
Claims priority of application No. 10-2015-0067872 (KR), filed on May 15, 2015; and application No. 10-2016-0059525 (KR), filed on May 16, 2016.
Prior Publication US 2021/0041359 A1, Feb. 11, 2021
Int. Cl. G01N 33/58 (2006.01); G01N 21/64 (2006.01)
CPC G01N 21/6428 (2013.01) [G01N 33/582 (2013.01); G01N 2021/6439 (2013.01)] 8 Claims
 
1. A system for detecting a biomaterial by means of a fluorophore having a linear upconversion fluorescent property, comprising:
a biomaterial diagnostic apparatus comprising an injection unit into which a biomaterial is injected and a reaction unit which includes a conjugate comprising a fluorophore and a molecule that binds the biomaterial, the fluorophore having a linear upconversion fluorescent property, wherein the conjuate forms a reaction complex with the biomaterial;
a light source unit configured to provide a light source to the reaction unit of the biomaterial diagnostic apparatus;
an emission signal collection unit configured to collect an emission signal with a wavelength range 10 to 100 nm shorter than that of excitation light exciting the fluorophore of the reaction complex; and
a measurement unit configured to detect and measure the collected emission signal,
wherein the light source unit provides a light source with a wavelength 5 to 50 nm longer than the maximum emission wavelength of the fluorophore, wherein the fluorophore has a Stokes' shift of <100 nm, and
wherein the fluorophore comprises a diketo pyrrolo pyrrole (DPP) derivative, wherein said DPP derivative is selected from the group consisting of 2,5-bis(2-ethylhexyl)-3,6-di(thiophen-2-yl)pyrrolo[3,4-c]pyrrole-1,4(2H,5H)-dione; 2,5-bis(2-ethylhexyl)-2,5-dihydro-3,6-diphenyl-pyrrolo[3,4-c]pyrrole-1,4-dione; 5-(2,5-bis(2-ethylhexyl)-1,2,4,5-tetrahydro-1,4-dioxo-3-(thiophen-2-yl)pyrrolo[3,4-c]pyrrol-6-yl)thiophene-2-carbaldehyde; and 5,5′-(2,5-bis(2-ethylhexyl)-3,6-dioxo-2,3,5,6-tetrahydropyrrolo[3,4-c]pyrrole-1,4-diyl)bis(thiophene-2-carbaldehyde).