	US 11,982,603 B2
	Preparation of biological cells on mass spectrometric sample supports for desorbing ionization
Katrin Sparbier, Bremen (DE); and Beatrix Wegemann, Bremen (DE)
Filed by Bruker Daltonics GmbH & Co. KG, Bremen (DE)
Filed on Mar. 23, 2022, as Appl. No. 17/702,276.
Application 17/702,276 is a continuation of application No. 15/968,428, filed on May 1, 2018, granted, now 11,307,122.
Claims priority of application No. 102017110476 (DE), filed on May 15, 2017.
Prior Publication US 2022/0276140 A1, Sep. 1, 2022
Int. Cl. G01N 1/40 (2006.01); C12Q 1/04 (2006.01); C12Q 1/18 (2006.01); G01N 1/28 (2006.01); G01N 33/68 (2006.01)

CPC G01N 1/4044 (2013.01) [C12Q 1/045 (2013.01); C12Q 1/18 (2013.01); G01N 1/28 (2013.01); G01N 33/6851 (2013.01); G01N 1/4055 (2013.01)]

18 Claims

1. A method for the preparation of proteins from samples of unpurified biological cells containing cell proteins on a sample support for the mass spectrometric determination of cellular properties, comprising the steps:

providing the biological cells on a sample spot of the sample support,

disrupting the cells and at least partially denaturing the cell proteins by using at least one of solvents and acids,

whereby causing the cell proteins to separate from complexes and to migrate out of the disrupted cells, and

whereby the cell proteins are to such an extent that they have sufficient reactive adhesion sites by which they adhere to a surface of the sample spot,

washing the cell proteins on the sample spot with buffer solution, and

preparing the washed cell proteins on the sample support for a subsequent desorbing ionization.