US 11,982,601 B2
Cell recovery method and device
Alexandre Izmailov, Etobicoke (CA); Brian Pak, Richmond Hill (CA); Tomoyuki Sen, Toronto (CA); and Paul Timothy Smith, Acton (CA)
Assigned to ANGLE EUROPE LIMITED, Guildford (GB)
Filed by ANGLE EUROPE LIMITED, Guildford (GB)
Filed on Feb. 27, 2023, as Appl. No. 18/114,506.
Application 17/868,977 is a division of application No. 17/840,373, filed on Jun. 14, 2022, granted, now 11,435,267, issued on Sep. 6, 2022.
Application 18/114,506 is a continuation of application No. 17/868,977, filed on Jul. 20, 2022, granted, now 11,604,119.
Prior Publication US 2023/0400392 A1, Dec. 14, 2023
Int. Cl. G01N 1/30 (2006.01); B01L 3/00 (2006.01)
CPC G01N 1/30 (2013.01) [B01L 3/502753 (2013.01); B01L 3/502761 (2013.01); B01L 2200/0647 (2013.01); B01L 2300/0822 (2013.01); B01L 2300/0832 (2013.01); B01L 2400/088 (2013.01)] 33 Claims
OG exemplary drawing
 
1. A method of cell sedimentation and retention of target cells, for example circulating tumour cells, CTC, from a fluid sample onto a solid support, the method comprising:
placing a fluid medium comprising the target cells in a fluid chamber, the fluid chamber having an open end sealed against a surface of the solid support;
subjecting the fluid medium to centrifugation within the fluid chamber to induce sedimentation of the target cells and promote cell adhesion to the surface of the solid support;
post-centrifugation, positioning a fluid absorbing element in the fluid chamber to remove fluid from the fluid chamber, and
controlling a flow rate of the fluid being absorbed by the fluid absorbing element such that the sedimented cells are not detached from the surface of the solid support.