US 11,982,268 B2
3D cell washer
Christopher Many, Saint Albans, VT (US); Brad Larson, South Burlington, VT (US); Byron Smith, South Hero, VT (US); and Brian Struhammer, Ferrisburg, VT (US)
Assigned to AGILENT TECHNOLOGIES, INC., Santa Clara, CA (US)
Filed by AGILENT TECHNOLOGIES, INC., Santa Clara, CA (US)
Filed on Mar. 29, 2021, as Appl. No. 17/215,606.
Application 17/215,606 is a division of application No. 15/911,865, filed on Mar. 5, 2018, granted, now 10,961,997.
Claims priority of provisional application 62/466,760, filed on Mar. 3, 2017.
Prior Publication US 2021/0301811 A1, Sep. 30, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 35/10 (2006.01); B05B 1/14 (2006.01); B08B 3/02 (2006.01); B08B 11/02 (2006.01); F04B 13/00 (2006.01); F04B 23/06 (2006.01); F04B 43/12 (2006.01); B01L 3/00 (2006.01); B01L 9/00 (2006.01); G01N 35/04 (2006.01)
CPC F04B 43/1253 (2013.01) [B01L 13/02 (2019.08); B05B 1/14 (2013.01); B08B 3/02 (2013.01); B08B 11/02 (2013.01); F04B 13/00 (2013.01); F04B 23/06 (2013.01); G01N 35/1002 (2013.01); G01N 35/1004 (2013.01); G01N 35/1009 (2013.01); B01L 3/5085 (2013.01); B01L 9/523 (2013.01); B01L 2300/0829 (2013.01); G01N 2035/0437 (2013.01); G01N 2035/1023 (2013.01)] 24 Claims
OG exemplary drawing
 
7. A microplate cell washing system comprising:
a microplate carrier configured to support a microplate, the microplate comprising a plurality of microplate wells;
a dispensing pump;
a dispensing pump cassette comprising a plurality of dispense lines connected to the dispensing pump and terminating in dispense pipes connected to the plurality of dispense lines, wherein the dispensing pump is configured to control a first flow rate of a solution from a fluid reservoir through the plurality of dispense lines;
an aspirating peristaltic pump;
an aspirating peristaltic pump cassette comprising a plurality of aspiration lines directly connected to the aspirating peristaltic pump and terminating in aspiration pipes connected to the plurality of aspiration lines, wherein the aspirating peristaltic pump is configured to control a second flow rate of a waste solution through the plurality of aspiration lines;
a processor configured to execute a cleaning control program, which when executed causes the microplate cell washing system to independently control:
the dispensing pump to control the first flow rate of the solution from the fluid reservoir through the plurality of dispense lines for output via the dispense pipes in microplate wells corresponding to positions of the dispense pipes, and
the aspirating peristaltic pump to control the second flow rate of the waste solution through the plurality of aspiration lines for aspiration via the aspiration pipes in microplate wells corresponding to positions of the aspiration pipes.