US 11,981,956 B2
Proximity detection methods and compositions
Sinem K. Saka, Cambridge, MA (US); Jocelyn Yoshiko Kishi, Cambridge, MA (US); and Peng Yin, Cambridge, MA (US)
Assigned to President and Fellows of Harvard College, Cambridge, MA (US)
Appl. No. 16/964,527
Filed by President and Fellows of Harvard College, Cambridge, MA (US)
PCT Filed Jan. 25, 2019, PCT No. PCT/US2019/015161
§ 371(c)(1), (2) Date Jul. 23, 2020,
PCT Pub. No. WO2019/147945, PCT Pub. Date Aug. 1, 2019.
Claims priority of provisional application 62/622,738, filed on Jan. 26, 2018.
Claims priority of provisional application 62/622,731, filed on Jan. 26, 2018.
Prior Publication US 2021/0147902 A1, May 20, 2021
Int. Cl. C12Q 1/682 (2018.01); C12Q 1/6804 (2018.01)
CPC C12Q 1/682 (2013.01) [C12Q 1/6804 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method of screening for a target molecule, the method comprising:
(a) contacting a first composition suspected of comprising a target molecule with a second composition, wherein the second composition comprises (i) a first target-binding molecule that binds specifically to a first epitope, (ii) a catalytic strand comprising a hairpin with a stem and a loop, wherein the catalytic strand binds to the first target-binding molecule, and wherein the catalytic strand comprises a molecule or modification that terminates polymerization, (iii) a second target-binding molecule that binds specifically to a second epitope, (iv) a concatemer-forming strand comprising a primer domain, wherein the primer domain is complementary to the stem, and the concatemer-forming strand binds to the second target-binding molecule, (v) a labeled imager strand that is complementary to the primer domain, (vi) a strand-displacing polymerase, and (vii) dNTPs; and
(b) detecting presence or absence of the labeled imager strand, wherein presence of the labeled imager strand indicates presence of the target molecule.