US 11,981,945 B2
Process of producing monoterpenes
Vijaykumar Karuppiah, Didcot (GB); Nicole G. H. Leferink, Manchester (GB); and Nigel S. Scrutton, Cheshire (GB)
Assigned to C3 BIOTECHNOLOGIES LIMITED, Lancaster (GB)
Filed by C3 Bio-Technologies Limited, Lancaster (GB)
Filed on Apr. 23, 2021, as Appl. No. 17/238,312.
Application 17/238,312 is a division of application No. 16/482,574, abandoned, previously published as PCT/GB2018/050223, filed on Jan. 26, 2018.
Claims priority of application No. 1701548 (GB), filed on Jan. 31, 2017.
Prior Publication US 2021/0238640 A1, Aug. 5, 2021
Int. Cl. C12P 17/06 (2006.01); C12N 9/88 (2006.01); C12P 7/04 (2006.01)
CPC C12P 17/06 (2013.01) [C12N 9/88 (2013.01); C12P 7/04 (2013.01); C12Y 402/03025 (2013.01); C12Y 402/03026 (2013.01); C12Y 402/03108 (2015.07)] 10 Claims
 
1. A process of producing linalool in a host microorganism comprising the following steps:
(a) providing a host microorganism genetically modified to express a bacterial monoterpene synthase (mTS), an Abies grandis geranyl diphosphate synthase, and a recombinant mevalonate-dependent (MVA) pathway comprising an E. coli acetoacetyl-CoA thiolase (AtoB), an S. aureus hydroxymethylglutaryl-CoA synthase (HMGS), an S. aureus hydroxymethylglutaryl-CoA reductase (HMGR), an S. cerevisiae mevalonate kinase (MK), an S. Cerevisiae phosphomevalonate kinase (PMK), an S. cerevisiae phosphomevalonate decarboxylase (PMD) and an E. coli isopentenyldiphosphate isomerase (idi);
(b) converting acetyl CoA into isopentenyl diphosphate (IPP) through the recombinant MVA pathway;
(c) converting IPP and dimethylallyl diphosphate (DMAPP) into geranyl pyrophosphate (GPP) through catalysis by the geranyl diphosphate synthase; and
(d) contacting GPP with said bacterial mTS to produce linalool,
wherein the bacterial mTS comprises Streptomyces clavuligerus linalool synthase,
wherein the host microorganism is a genetically modified bacterium comprising E. coli, and
wherein the process results in a linalool yield of at least 300 mg Lorg−1.