US 11,981,943 B2
Methods and composition for detecting CAPN5
Vinit B. Mahajan, Iowa City, IA (US); Alexander G. Bassuk, Iowa City, IA (US); Diana F. Colgan, Iowa City, IA (US); Gabriel Velez, Iowa City, IA (US); Kellie Schaefer, Iowa City, IA (US); Marcus Toral, Iowa City, IA (US); Shu Wu, Iowa City, IA (US); and Lokesh Gakhar, Iowa City, IA (US)
Assigned to UNIVERSITY OF IOWA RESEARCH FOUNDATION, Iowa City, IA (US)
Appl. No. 16/340,657
Filed by UNIVERSITY OF IOWA RESEARCH FOUNDATION, Iowa City, IA (US)
PCT Filed Oct. 2, 2017, PCT No. PCT/US2017/054727
§ 371(c)(1), (2) Date Apr. 9, 2019,
PCT Pub. No. WO2018/071216, PCT Pub. Date Apr. 19, 2018.
Claims priority of provisional application 62/406,806, filed on Oct. 11, 2016.
Claims priority of provisional application 62/525,510, filed on Jun. 27, 2017.
Prior Publication US 2019/0284544 A1, Sep. 19, 2019
Int. Cl. C12N 9/64 (2006.01); A61K 9/08 (2006.01); A61K 38/00 (2006.01); A61P 27/02 (2006.01); A61P 29/00 (2006.01); C07K 14/49 (2006.01); C07K 14/81 (2006.01); G01N 33/573 (2006.01); C07K 7/64 (2006.01)
CPC C12N 9/6472 (2013.01) [A61K 9/08 (2013.01); A61P 27/02 (2018.01); A61P 29/00 (2018.01); C07K 14/49 (2013.01); C07K 14/81 (2013.01); C07K 14/8139 (2013.01); C12Y 304/22 (2013.01); G01N 33/573 (2013.01); A61K 38/00 (2013.01); C07K 7/64 (2013.01); C07K 2319/00 (2013.01)] 14 Claims
 
1. A labelled CAPN5 substrate consisting of
a peptide selected from the group consisting of SEQ ID NO:20 and not more than 9 additional amino acids, SEQ ID NO:21 and not more than 9 additional amino acids, or SEQ ID NO:23 and not more than 19 additional amino acids, and
a detection agent consisting of a fluorescent or colorimetric agent to form the labelled CAPN5 substrate.