US 11,981,934 B2
Adeno-associated virus purification methods
Christian Fiedler, Vienna (AT); Meinhard Hasslacher, Vienna (AT); and Jadranka Koehn, Laupheim (DE)
Assigned to TAKEDA PHARMACEUTICAL COMPANY LIMITED, Osaka (JP)
Appl. No. 16/958,974
Filed by TAKEDA PHARMACEUTICAL COMPANY LIMITED, Osaka (JP)
PCT Filed Dec. 27, 2018, PCT No. PCT/US2018/067627
§ 371(c)(1), (2) Date Jun. 29, 2020,
PCT Pub. No. WO2019/133677, PCT Pub. Date Jul. 4, 2019.
Claims priority of provisional application 62/611,709, filed on Dec. 29, 2017.
Prior Publication US 2020/0332266 A1, Oct. 22, 2020
Int. Cl. C07K 14/435 (2006.01); A61K 38/00 (2006.01); B01D 15/16 (2006.01); B01D 15/36 (2006.01); B01D 15/38 (2006.01); B01D 15/42 (2006.01); C07K 14/705 (2006.01); C07K 16/28 (2006.01); C12N 7/00 (2006.01); G01N 33/569 (2006.01)
CPC C12N 7/00 (2013.01) [B01D 15/166 (2013.01); B01D 15/363 (2013.01); B01D 15/3809 (2013.01); B01D 15/426 (2013.01); G01N 33/56983 (2013.01); C12N 2750/14151 (2013.01); G01N 2333/075 (2013.01)] 29 Claims
 
1. A method of purifying an adeno-associated virus (AAV) comprising
(a) loading an AAV containing solution onto an affinity resin targeted against AAV under conditions that allow binding between the AAV in the solution and the affinity resin;
(b) undertaking at least two wash steps; and
(c) eluting the AAV from the affinity resin;
wherein at least one of the wash steps comprises applying to the affinity resin a buffer comprising an organic solvent or detergent.