a) contacting and culturing an isolated population of cells comprising γδ T cells with an activation culture to activate and enrich Vγ9Vδ2 γδ T cells within the isolated population of cells; wherein the activation culture comprises IL-15 and zoledronic acid;

b) after step (a), purifying the Vγ9Vδ2-enriched γδ T cells from the isolated population of cells;

c) transducing the purified Vγ9Vδ2-enriched γδ T cells with a viral vector encoding one or more reprogramming factors; and

d) culturing the transduced cells under conditions suitable for reprogramming mammalian somatic cells to a pluripotent state, thereby producing iPSCs;

wherein the produced iPSCs have rearranged TRG and TRD genes;

wherein the produced iPSCs have TCR rearrangements containing Vγ9 and Vδ2 gene segments;

wherein optionally the activation culture further comprises IL-2;

wherein optionally the viral vector is a Sendai virus (SeV) vector;

wherein optionally the method further comprises obtaining the isolated population of cells from a subject prior to step (a); and

wherein optionally the isolated population of cells are peripheral blood mononuclear cells (PBMCs).