US 11,981,904 B2
BAHD acyltransferases
Steven D. Karlen, Madison, WI (US); Rebecca Anne Smith, Madison, WI (US); John Ralph, Madison, WI (US); Emily Beebe, Stoughton, WI (US); Craig Bingman, Fitchburg, WI (US); Brian Fox, Madison, WI (US); Shawn Mansfield, Vancouver (CA); Heather Mackay, Vancouver (CA); Hoon Kim, Madison, WI (US); Yaseen Mottiar, Vancouver (CA); and Faride Unda, Vancouver (CA)
Assigned to Wisconsin Alumni Research Foundation, Madison, WI (US); and The University of British Columbia, Vancouver (CA)
Appl. No. 17/291,932
Filed by WISCONSIN ALUMNI RESEARCH FOUNDATION, Madison, WI (US); and THE UNIVERSITY OF BRITISH COLUMBIA, Vancouver (CA)
PCT Filed Nov. 8, 2019, PCT No. PCT/US2019/060554
§ 371(c)(1), (2) Date May 6, 2021,
PCT Pub. No. WO2020/097518, PCT Pub. Date May 14, 2020.
Claims priority of provisional application 62/757,804, filed on Nov. 9, 2018.
Prior Publication US 2022/0002744 A1, Jan. 6, 2022
Int. Cl. C12N 15/82 (2006.01); C07K 14/41 (2006.01); C07K 14/415 (2006.01); C12N 9/10 (2006.01)
CPC C12N 15/8255 (2013.01) [C07K 14/415 (2013.01); C12N 9/1029 (2013.01); C12N 15/8223 (2013.01); C12Y 203/01196 (2013.01)] 63 Claims
 
1. A recombinant nucleic acid comprising a nucleic acid segment that encodes:
a BAHD acyltransferase polypeptide with at least 95% amino acid sequence identity to SEQ ID NO:2 that exhibits p-hydroxybenzoyl-CoA:monolignol transferase (pBMT) activity, feruloyl-CoA:monolignol transferase (FMT) activity, p-coumaroyl-CoA:monolignol transferase (PMT) activity, acetyl-CoA:monolignol transferase (AMT) activity, benzoyl-CoA:monolignol transferase (BMT) activity, or any combination thereof;
a BAHD acyltransferase polypeptide with at least 95% amino acid sequence identity to SEQ ID NO:4 that exhibits pBMT activity, AMT activity, BMT activity, or any combination thereof;
a BAHD acyltransferase polypeptide with at least 95% amino acid sequence identity to SEQ ID NO:6 that exhibits pBMT activity, AMT activity, BMT activity, or any combination thereof;
a BAHD acyltransferase polypeptide with at least 95% amino acid sequence identity to SEQ ID NO:8 that exhibits FMT activity, PMT activity, BMT activity, or any combination thereof;
a BAHD acyltransferase polypeptide with at least 95% amino acid sequence identity to SEQ ID NO:12 that exhibits pBMT activity;
a BAHD acyltransferase polypeptide with at least 95% amino acid sequence identity to SEQ ID NO:14 that exhibits FMT activity, PMT activity, or any combination thereof;
a BAHD acyltransferase polypeptide with at least 95% amino acid sequence identity to SEQ ID NO:16 that exhibits FMT activity, PMT activity, or any combination thereof; or
a BAHD acyltransferase polypeptide with at least 95% amino acid sequence identity to SEQ ID NO:18 that exhibits FMT activity,
wherein the nucleic acid segment is operably linked to a heterologous promoter, wherein the promoter is a promoter functional or active during plant development or growth.