	US 11,981,892 B2
	Compositions and methods for improved gene editing
Craig Cameron Mello, Barrington, RI (US); Krishna Sumanth Ghanta, Grafton, MA (US); Gregoriy Dokshin, Belmont, MA (US); Aamir Mir, Worcester, MA (US); Hassan Gneid, Worcester, MA (US); Jonathan Kenneth Watts, Worcester, MA (US); and Erik Joseph Sontheimer, Auburndale, MA (US)
Assigned to UNIVERSITY OF MASSACHUSETTS, Boston, MA (US)
Filed by UNIVERSITY OF MASSACHUSETTS, Boston, MA (US)
Filed on Apr. 15, 2019, as Appl. No. 16/384,612.
Claims priority of provisional application 62/679,315, filed on Jun. 1, 2018.
Claims priority of provisional application 62/658,368, filed on Apr. 16, 2018.
Prior Publication US 2019/0345479 A1, Nov. 14, 2019
Int. Cl. C12N 15/10 (2006.01); C12N 9/22 (2006.01); C12N 15/113 (2010.01); C12N 15/66 (2006.01); C12N 15/90 (2006.01)

CPC C12N 15/102 (2013.01) [C12N 9/22 (2013.01); C12N 15/113 (2013.01); C12N 15/66 (2013.01); C12N 15/902 (2013.01)]

39 Claims

1. A genome-editing system comprising:

i) an isolated nucleic acid donor molecule comprising

(a) a region having portions of nucleic acid homology to a target sequence; and

(b) one or more terminal adaptors comprising an ethylene glycol or a polyethylene glycol (PEG);

wherein the nucleic acid donor molecule length is 100 nucleotides or more,

wherein the one or more terminal adaptors are attached to the 5′ end and/or the 3′ end of the nucleic acid donor molecule; and

wherein the nucleic acid donor molecule is capable of being introduced into a target sequence by a homology-directed repair (HDR) or homology-independent targeted integration (HITI) mechanism; and

ii) an agent that creates a double-stranded break at or near the target sequence.