US 11,980,699 B2
Cartilage regeneration using injectable, in situ polymerizable collagen compositions containing chondrocytes or stem cells
Dale P. DeVore, Chelmsford, MA (US); and Jiaxun Zhu, Shanghai (CN)
Assigned to Shanghai Qisheng Biological Preparation Co., Ltd., Shanghai (CN)
Filed by Shanghai Qisheng Biological Preparation Co., Ltd., Shanghai (CN)
Filed on Sep. 1, 2022, as Appl. No. 17/901,258.
Claims priority of provisional application 63/239,496, filed on Sep. 1, 2021.
Prior Publication US 2023/0083186 A1, Mar. 16, 2023
Int. Cl. A61L 27/24 (2006.01); A61K 47/18 (2017.01); A61L 27/38 (2006.01); A61L 27/36 (2006.01)
CPC A61L 27/3852 (2013.01) [A61L 27/24 (2013.01); A61L 27/3817 (2013.01); A61L 27/3834 (2013.01); A61L 27/3687 (2013.01); A61L 2430/06 (2013.01)] 21 Claims
OG exemplary drawing
 
1. An in situ polymerizable collagen-based injectable composition for cartilage defect repair or treatment by injection comprising:
(i) an injectable, in situ polymerizable collagen composition comprising: a neutralized solution comprising acid soluble collagen, EDTA or EGTA and a polyol, wherein the acid soluble collagen in the solution has not undergone fibrillogenesis prior to injection and is selected from one or more of Type I collagen, Type II collagen, and Type III collagen, wherein said acid soluble collagen is in a concentration of 5 to 70 mg/ml; and
(ii) chondrocytes or stem cells comprised in part (i), wherein the in situ polymerizable collagen composition serves as an injectable scaffold supporting cell proliferation; and
(iii) optionally, substance(s) for enhancing cartilage defect repair or treatment and/or for enhancing cell proliferation and function; and/or
(iv) optionally, a slowly resorbable scaffold or micro-carriers,
wherein the in situ polymerizable collagen-based injectable composition with cellular components is in a form that rapidly undergo gelation and polymerization when contacted by physiological fluids and serves as a scaffold supporting cell proliferation.