	US 11,980,672 B2
	Heterologous UTR sequences for enhanced mRNA expression
David H. Burkhardt, Somerville, MA (US); Romesh R. Subramanian, Framingham, MA (US); and Christian Cobaugh, Newton Highlands, MA (US)
Assigned to ModernaTX, Inc., Cambridge, MA (US)
Filed by ModernaTX, Inc., Cambridge, MA (US)
Filed on Jun. 21, 2022, as Appl. No. 17/844,877.
Application 17/844,877 is a division of application No. 15/781,827, granted, now 11,389,546, previously published as PCT/US2016/065796, filed on Dec. 9, 2016.
Claims priority of provisional application 62/265,233, filed on Dec. 9, 2015.
Prior Publication US 2023/0050143 A1, Feb. 16, 2023
Int. Cl. A61K 48/00 (2006.01); A61K 31/7115 (2006.01); A61K 31/712 (2006.01); A61K 31/7125 (2006.01); A61P 1/16 (2006.01); A61P 31/14 (2006.01); C12N 15/10 (2006.01); C12N 15/67 (2006.01); C12Q 1/68 (2018.01)

CPC A61K 48/0058 (2013.01) [A61K 31/7115 (2013.01); A61K 31/712 (2013.01); A61K 31/7125 (2013.01); A61K 48/0066 (2013.01); A61P 1/16 (2018.01); A61P 31/14 (2018.01); C12N 15/1086 (2013.01); C12N 15/67 (2013.01); C12N 2840/00 (2013.01)]

18 Claims

1. A messenger RNA (mRNA) comprising an open reading frame (ORF) encoding protein, wherein the ORF is flanked by a heterologous 5′ untranslated region (UTR) and/or a heterologous 3′ UTR, wherein the 5′ UTR and/or the 3′ UTR are derived from a gene selected from the group consisting of Haptoglobin (HP), Haptoglobin-Related Protein (HPR), Complement Component 3 (C3), Alpha-1-antitrypsin (SERPINA1), Alpha-1-antichymotrypsin (SERPINA3), Fibrinogen Alpha Chain (FGA), and Fibrinogen Beta Chain (FGB).