| CPC G06T 7/33 (2017.01) [G01N 21/6456 (2013.01); G06T 7/38 (2017.01); G16H 30/40 (2018.01); G06T 2207/10028 (2013.01); G06T 2207/10048 (2013.01); G06T 2207/10064 (2013.01); G06T 2207/10081 (2013.01); G06T 2207/10088 (2013.01); G06T 2207/10101 (2013.01); G06T 2207/10136 (2013.01); G06T 2207/30024 (2013.01); G06T 2207/30096 (2013.01); G06T 2207/30204 (2013.01)] | 85 Claims |

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1. A method of generating a three-dimensional image of a target using two-dimensional images obtained within an enclosed environment, comprising:
while a target is positioned within the enclosed environment:
generating a three-dimensional map of a target area associated with one or more fiducial markers;
capturing a two-dimensional white light image of the target area and the one or more fiducial markers;
capturing a two-dimensional fluorescence image of the target area and the one or more fiducial markers;
creating a three-dimensional white light image from the two-dimensional white light image and the three-dimensional map;
creating a three-dimensional fluorescence image from the two-dimensional fluorescence image and the three-dimensional map; and
aligning the three-dimensional white light image and the three-dimensional fluorescence image using the one or more fiducial markers to form a three-dimensional superimposed image.
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