US 12,298,320 B2
Compound distribution in microfluidic devices
Josiah Sliz, Boston, MA (US); Daniel Levner, Brookline, MA (US); Brian Zuckerman, Cambridge, MA (US); Norman Wen, West Roxbury, MA (US); Jonathan Rubins, Cambridge, MA (US); Tanvi Shroff, Cambridge, MA (US); Christopher David Hinojosa, Malden, MA (US); Grace Ahn, Medford, MA (US); Victor Antontsev, Somerville, MA (US); Jefferson Puerta, Malden, MA (US); David Conegliano, Boston, MA (US); and S. Jordan Kerns, Reading, MA (US)
Assigned to EMULATE, INC., Boston, MA (US)
Filed by EMULATE, Inc., Boston, MA (US)
Filed on Nov. 30, 2021, as Appl. No. 17/538,518.
Application 17/538,518 is a continuation of application No. PCT/US2020/039830, filed on Jun. 25, 2020.
Claims priority of provisional application 62/867,543, filed on Jun. 27, 2019.
Prior Publication US 2022/0155328 A1, May 19, 2022
Int. Cl. G01N 35/00 (2006.01); B01L 3/00 (2006.01); C12Q 3/00 (2006.01); G01N 15/06 (2024.01); G01N 33/50 (2006.01)
CPC G01N 35/00584 (2013.01) [B01L 3/502761 (2013.01); C12Q 3/00 (2013.01); G01N 15/06 (2013.01); G01N 33/5008 (2013.01); B01L 3/502707 (2013.01); B01L 2200/0647 (2013.01); B01L 2200/148 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/069 (2013.01); B01L 2300/10 (2013.01); B01L 2400/0406 (2013.01); G01N 2035/00544 (2013.01); G01N 2035/0097 (2013.01)] 17 Claims
OG exemplary drawing
 
1. A method of analyzing compound distribution in a system, comprising:
a) providing i) a first system comprising a first microfluidic device comprising a first membrane with pores in a plurality of regions, and ii) a first experimental protocol for use with said first system, said first experimental protocol comprising introducing a compound into said first microfluidic device and taking actions at one or more timepoints;
b) replacing said first system with a second system, said second system comprising a second microfluidic device comprising a second membrane without pores in at least one region in which said first membrane comprises pores;
c) modifying said first experimental protocol to generate a modified experimental protocol, wherein said modified experimental protocol comprises introducing said compound into said second microfluidic device and measuring compound concentration at one or more of said timepoints from said first experimental protocol;
d) performing said modified experimental protocol; and
e) using said measurement of concentration of said compound to analyze compound distribution across said system.