US 12,298,313 B1
Methods for detecting AAV
Xiaoying Jin, Cambridge, MA (US); Catherine O'Riordan, Cambridge, MA (US); Lin Liu, Cambridge, MA (US); and Kate Zhang, Cambridge, MA (US)
Assigned to Genzyme Corporation, Cambridge, MA (US)
Filed by Genzyme Corporation, Cambridge, MA (US)
Filed on Jan. 8, 2025, as Appl. No. 19/013,863.
Application 18/801,293 is a division of application No. 18/321,542, filed on May 22, 2023, granted, now 12,123,880, issued on Oct. 22, 2024.
Application 18/321,542 is a division of application No. 16/325,653, granted, now 11,698,377, issued on Jul. 11, 2023, previously published as PCT/US2017/046814, filed on Aug. 14, 2017.
Application 19/013,863 is a continuation of application No. 18/801,293, filed on Aug. 12, 2024.
Claims priority of provisional application 62/375,314, filed on Aug. 15, 2016.
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/68 (2006.01); C12N 15/86 (2006.01); G01N 30/72 (2006.01); G01N 30/02 (2006.01)
CPC G01N 33/6848 (2013.01) [C12N 15/86 (2013.01); G01N 30/72 (2013.01); C12N 2750/14123 (2013.01); C12N 2750/14143 (2013.01); G01N 2030/027 (2013.01)] 27 Claims
OG exemplary drawing
 
1. A method of detecting post-translational modifications of one or more viral proteins (VPs) in a preparation of adeno-associated virus (AAV) particles, the method comprising
a) denaturing the AAV particles;
b) subjecting the denatured AAV particles to liquid chromatography/mass spectrometry (LC/MS) intact protein analysis;
c) determining the masses of the one or more VPs; and
d) determining any deviation of the determined masses of the one or more VPs from the theoretical masses of corresponding VPs that have not undergone post-translational modifications to detect a deviation in the compared masses,
wherein the VPs comprise VP1, VP2 and VP3 capsid proteins, and wherein the method is performed in the absence of a gel separation step.