US 12,298,300 B2
Single-molecule electronic sequence detector and methods of use
Stuart Lindsay, Scottsdale, AZ (US); Karen Anderson, Scottsdale, AZ (US); Mark Knappenberger, Scottsdale, AZ (US); Sepideh Afsari Mamaghani, Scottsdale, AZ (US); and Jacob L. Swett, Tempe, AZ (US)
Assigned to Arizona Board of Regents on behalf of Arizona State University, Scottsdale, AZ (US); and Recognition Analytix, Inc., Phoenix, AZ (US)
Filed by ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY, Scottsdale, AZ (US); and RECOGNITION ANALYTIX INC., Phoenix, AZ (US)
Filed on Apr. 16, 2021, as Appl. No. 17/232,631.
Claims priority of provisional application 63/011,799, filed on Apr. 17, 2020.
Prior Publication US 2021/0325379 A1, Oct. 21, 2021
Int. Cl. G01N 33/53 (2006.01); C12N 9/22 (2006.01); C12N 15/11 (2006.01); G01N 27/02 (2006.01)
CPC G01N 33/5308 (2013.01) [C12N 9/22 (2013.01); C12N 15/11 (2013.01); G01N 27/021 (2013.01); C12N 2310/20 (2017.05); C12N 2800/80 (2013.01)] 27 Claims
OG exemplary drawing
 
1. A bioelectronic device for detecting a target nucleic acid comprising:
a first electrode;
a second electrode; and
at least one CRISPR-associated protein comprising a modification to form a chemical bond with the first and the second electrodes, wherein the modification comprises attaching at least two linkers to the CRISPR-associated protein, and wherein at least one of the at least two linkers is attached to each of the first and second electrodes.