US 12,298,286 B2
Method for separation and quantitation of benzene, toluene, ethyl benzene, o-xylene, m-xylene, and p-xylene (BTEX) using high performance liquid chromatography
Saroj Kumar Panda, Dhahran (SA); Amal Lajami, Qatif (SA); Robert Peter Hodgkins, Dhahran (SA); and Omer Refa Koseoglu, Dhahran (SA)
Assigned to Saudi Arabian Oil Company, Dhahran (SA)
Filed by SAUDI ARABIAN OIL COMPANY, Dhahran (SA)
Filed on Jun. 24, 2022, as Appl. No. 17/849,249.
Prior Publication US 2023/0417715 A1, Dec. 28, 2023
Int. Cl. G01N 30/88 (2006.01); G01N 30/86 (2006.01)
CPC G01N 30/88 (2013.01) [G01N 30/8665 (2013.01); G01N 2030/884 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A method for the determination of concentrations of aromatic hydrocarbon components in industrial samples, the method comprising the steps of:
injecting a prepared sample into a high-performance liquid chromatography column, wherein the high-performance liquid chromatography column comprises a carbon stationary phase, further wherein the prepared sample comprises the aromatic hydrocarbon components, wherein the aromatic hydrocarbon components are selected from the group consisting of: benzene, toluene, ethyl benzene, xylene isomers, and combinations of the same, wherein the xylene isomers are selected from the group consisting of: o-xylene, p-xylene, m-xylene, and combinations of the same;
introducing a mobile phase into the carbon stationary phase, wherein the mobile phase comprises an organic acid in water and an alcohol;
increasing the concentration of the alcohol in the mobile phase and decreasing the concentration of the organic acid in water in the mobile phase such that the mobile phase reaches a concentration of 100% of the alcohol and 0% of the organic acid in water, such that a gradient elution is performed; then
decreasing the concentration of the alcohol in the mobile phase and increasing the concentration of the organic acid in water in the mobile phase; and
quantitating, based on the gradient elution, a concentration of the xylene isomers in the prepared sample.