US 12,298,230 B2
Detection method
José Pedro Santos Manso Côrte-Real, Lisbon (PT); José Miguel Silva Do Carmo Nogueira, Barreiro (PT); André Miguel Alves Farinha, Seixal (PT); Alberto Eduardo Artilheiro Ferreira, Lisbon (PT); Tiago Filipe Pinto Silva, Lisbon (PT); Mauro Miguel Melo Ribeiro, Apelação (PT); Filipe Da Conceição Fernandes Thomaz, Lisbon (PT); and João Manuel De Oliveira Garcia Da Fonseca, Azambuja (PT)
Assigned to BIOSURFIT, S.A., Azambuja (PT)
Appl. No. 16/644,770
Filed by BIOSURFIT, S.A., Azambuja (PT)
PCT Filed Sep. 4, 2018, PCT No. PCT/EP2018/073744
§ 371(c)(1), (2) Date Mar. 5, 2020,
PCT Pub. No. WO2019/048429, PCT Pub. Date Mar. 14, 2019.
Claims priority of application No. 110277 J (PT), filed on Sep. 5, 2017; and application No. 17191770 (EP), filed on Sep. 19, 2017.
Prior Publication US 2021/0063303 A1, Mar. 4, 2021
Int. Cl. G01N 21/27 (2006.01); G01N 21/03 (2006.01); G01N 21/07 (2006.01); G01N 21/75 (2006.01); G01N 35/00 (2006.01); G01N 35/02 (2006.01); G01N 35/04 (2006.01)
CPC G01N 21/272 (2013.01) [G01N 21/07 (2013.01); G01N 21/75 (2013.01); G01N 35/00623 (2013.01); G01N 35/025 (2013.01); G01N 2021/0357 (2013.01); G01N 2035/00841 (2013.01); G01N 2035/00851 (2013.01); G01N 2035/0444 (2013.01)] 15 Claims
OG exemplary drawing
 
1. A method of determining an effect of a liquid sample on light, the method comprising:
rotating an assay device comprising one or more chambers for containing the liquid sample;
directing light from a light source to the rotating assay device such that the light passes through the one or more chambers as each of the one or more chambers is in a respective predetermined configuration relative to the light source and a light receiver, and wherein the light source and the light receiver are arranged on the same side of the rotating assay device;
receiving, at the light receiver, light from the light source that has passed through the one or more chambers within the rotating assay device for at least one complete rotation of the assay device;
determining a set of light intensity measurements for each complete rotation of the assay device;
determining measurements of light intensity within each set of light intensity measurements that were obtained when the light passed through the one or more chambers;
determining the number of measurements that meet a light intensity condition in one of the sets of measurements;
changing the light intensity condition for sets of measurements for subsequent complete rotations based on the number of measurements that met the light intensity condition; and
determining, using the measurements of light intensity that were obtained when the light passed through the one or more chambers, the effect of the liquid sample on the light from the light source.