	US 12,297,440 B2
	Regulatory nucleic acid molecules for enhancing gene expression in plants
Frank Meulewaeter, Ghent (BE); Shirong Zhang, Morrisville, NC (US); and Christophe Liseron-Monfils, Ghent (BE)
Assigned to BASF SE, Ludwigshafen am Rhein (DE)
Appl. No. 17/609,966
Filed by BASF SE, Ludwigshafen (DE)
PCT Filed May 6, 2020, PCT No. PCT/EP2020/062488 § 371(c)(1), (2) Date Nov. 9, 2021, PCT Pub. No. WO2020/229241, PCT Pub. Date Nov. 19, 2020.
Claims priority of application No. 19173869 (EP), filed on May 10, 2019.
Prior Publication US 2022/0220495 A1, Jul. 14, 2022
Int. Cl. C12N 15/82 (2006.01)

CPC C12N 15/8234 (2013.01)

11 Claims

1. A method for enhancing expression derived from a plant promoter comprising functionally linking to a promoter one or more nucleic acid expression enhancing nucleic acid (NEENA) molecules heterologous to said promoter, wherein the one or more NEENA molecules consists of the sequence of SEQ ID NO: 2, wherein enhancer activity of the one or more NEENA molecules is independent of the orientation of the one or more NEENA molecules with respect to the promoter.