	US 12,297,430 B2
	O-methyl rich fully stabilized oligonucleotides
Anastasia Khvorova, Westborough, MA (US); Julia Alterman, Worcester, MA (US); Sarah Davis, Boston, MA (US); and Anton Turanov, Boston, MA (US)
Assigned to UNIVERSITY OF MASSACHUSETTS, Westborough, MA (US)
Filed by UNIVERSITY OF MASSACHUSETTS, Boston, MA (US)
Filed on Jan. 20, 2022, as Appl. No. 17/580,269.
Application 17/580,269 is a continuation of application No. 16/550,076, filed on Aug. 23, 2019, granted, now 11,279,930.
Claims priority of provisional application 62/721,993, filed on Aug. 23, 2018.
Prior Publication US 2022/0251555 A1, Aug. 11, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/113 (2010.01)

CPC C12N 15/113 (2013.01) [C12N 2310/14 (2013.01); C12N 2310/315 (2013.01); C12N 2310/321 (2013.01); C12N 2310/322 (2013.01); C12N 2310/52 (2013.01)]

20 Claims

1. An oligonucleotide comprising,

at least 14 contiguous nucleotides, a 5′ end and a 3′ end; and

at least 85% 2′-O-methyl nucleotide modifications,

wherein the nucleotides at positions 2, 14, and 20 from the 5′ end of the oligonucleotide comprise a non-2′-O-methyl modification.