US 12,297,424 B2
Pseudomonas fluorescens N1 and use thereof
Yu Zhao, Beijing (CN); Ying Zhang, Beijing (CN); Tiegen Zhang, Beijing (CN); and Changxi Gong, Beijing (CN)
Assigned to SHANXI BOYU BIOTECHNOLOGY CO., LTD., Taiyuan (CN)
Filed by SHANXI BOYU BIOTECHNOLOGY CO., LTD., Taiyuan (CN)
Filed on Jun. 24, 2024, as Appl. No. 18/752,770.
Application 18/752,770 is a continuation of application No. PCT/CN2022/126107, filed on Oct. 19, 2022.
Claims priority of application No. 202111251300 (CN), filed on Oct. 26, 2021; and application No. 202210116969 (CN), filed on Feb. 7, 2022.
Prior Publication US 2024/0368537 A1, Nov. 7, 2024
Int. Cl. C12N 1/20 (2006.01); B09B 3/60 (2022.01); B09C 1/10 (2006.01); C05B 13/02 (2006.01); C05F 7/00 (2006.01); C05F 17/20 (2020.01); C12R 1/39 (2006.01)
CPC C12N 1/205 (2021.05) [B09B 3/60 (2022.01); B09C 1/10 (2013.01); C05B 13/02 (2013.01); C05F 7/00 (2013.01); C05F 17/20 (2020.01); C12R 2001/39 (2021.05)] 3 Claims
 
1. A method for modifying solid waste by a microorganism synergistically with coal gangue material, comprising the following steps:
(1) adding coal gangue to magnesium slag in an amount of 10-15% by volume of the magnesium slag, mixing thoroughly, sieving to control a particle size of the mixed material to be 2-5 mm, and stacking for 5-10 days;
(2) adding vinegar dregs in an amount of 20-30% by volume of the magnesium slag, mixing thoroughly, and adjusting the pH value to 5.5-6.5 using a wood vinegar liquid; and
(3) spraying Pseudomonas fluorescens N1 registered in the China General Microbiological Culture Collection Center under the accession number CGMCC No. 23192 or Pseudomonas fluorescens N1 registered in the China General Microbiological Culture Collection Center under the accession number CGMCC No. 23192+Xanthomonas campestris mixed in an equal solution volume onto the mixed material surface, and spraying water in an amount of 0.50-1.00 m3/m3 mixed material per day for 30-40 days.