US 12,297,422 B2
Controlled growth of microorganisms
Philippe Gabant, Ottignies Louvain-la-Neuve (BE)
Assigned to Syngulon SA, Seraing (BE)
Filed by Syngulon SA, Seraing (BE)
Filed on Aug. 26, 2022, as Appl. No. 17/822,663.
Application 15/087,706 is a division of application No. 14/459,810, filed on Aug. 14, 2014, granted, now 9,333,227, issued on May 10, 2016.
Application 17/822,663 is a continuation of application No. 16/227,371, filed on Dec. 20, 2018, granted, now 11,427,800.
Application 16/227,371 is a continuation of application No. 15/087,706, filed on Mar. 31, 2016, granted, now 10,188,114, issued on Jan. 29, 2019.
Claims priority of provisional application 61/867,510, filed on Aug. 19, 2013.
Prior Publication US 2023/0193191 A1, Jun. 22, 2023
Int. Cl. C12N 1/12 (2006.01); A01N 63/50 (2020.01); A61K 35/74 (2015.01); A61K 36/02 (2006.01); A61K 36/06 (2006.01); C07K 14/195 (2006.01); C12N 1/16 (2006.01); C12N 1/20 (2006.01); C12N 1/38 (2006.01)
CPC C12N 1/12 (2013.01) [A01N 63/50 (2020.01); A61K 35/74 (2013.01); A61K 36/02 (2013.01); A61K 36/06 (2013.01); C07K 14/195 (2013.01); C12N 1/16 (2013.01); C12N 1/20 (2013.01); C12N 1/38 (2013.01)] 31 Claims
 
1. A method of controlling the growth of a microbial cell, the method comprising:
culturing a genetically engineered microbial cell under conditions in which the genetically engineered microbial cell produces a secreted bacteriocin at a level sufficient to control the growth of the genetically engineered microbial cell and/or a second microbial cell,
wherein said genetically engineered microbial cell comprises:
a first nucleic acid comprising a first nucleic acid sequence encoding the secreted bacteriocin that is capable of inhibiting or preventing reproduction of said genetically engineered microbial cell and/or second microbial cell in the absence of an immunity modulator that confers resistance to said secreted bacteriocin; and
a second nucleic acid comprising a second nucleic acid sequence that encodes the immunity modulator that confers resistance to said secreted bacteriocin, wherein the second nucleic acid sequence is on a chromosome, and
wherein said genetically engineered microbial cell has been genetically engineered to conditionally decrease or eliminate at least one of transcription, post-transcriptional expression or post-transcriptional activity of said immunity modulator concurrent with expression of the secreted bacteriocin, thereby causing the secreted bacteriocin to inhibit or prevent reproduction of the genetically engineered microbial cell.